Plasmids and phages

Cioning via YACs, BACs, cosmids, phages, and plasmids Permits isolation of fragments of varying lengths. 

H. Miller, Methods Enzymol. 152, 145-172 (1987). Practical Aspects of Preparing Phage and Plasmid DNA Growth, Maintenance and Storage of Bacteria and Bacteriophage. ... 

Guest, J. R., 1981, Partial replacement of succinate dehydrogenase function hy phage- and plasmid-specified fumarate reductase in Escherichia coli, J. Gen. Microbiol. 122 1719179. 

Know what cloning vectors (plasmid, phage, and cosmids) are and how they are used to insert foreign DNA into a cell. 

The Y2- Rep and Relaxase proteins are less well nnderstood, and the Y2 IS9i transposase is proving to be difficult to work with. Again, it is assumed largely that the Y2 enzyme behaves the same as the related plasmid and phage Rep and plasmid Relaxase proteins. The mechanism of the final group, the Y1 transposases, is now relatively well understood. 

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