DNA mapping

Workers in the early 1970s recognized that restriction enzymes provided tools not only for DNA mapping but also for constmction of new DNA species not found in nature. A collection of recombinant DNA species consisting of many passenger sequences joined to identical vector molecules is called a hbrary. Individual recombinant DNAs are isolated from single clones of the Hbrary for detailed analysis and manipulation. 

Chetrit, P., Gaudin, V., de Courcel, A., and Vedel, F. (1989) A cross-hybridization method for DNA mapping with photobiotin-labeled probes. Anal. Biochem. 178, 273-275. 

Farnsworth NR, Akerele O, Bingel AS, Soejarto DD, Guo Z. (1985) Can variola-like viruses be derived from monkeypox virus An investigation based on DNA mapping. Bull WHO 63 695-981. 

Mclnnes et al., 1987), for the diagnosis of barley yellow dwarf virus (Habili et al., 1987), to assay luteinizing hormone (3 mRNA in individual gonadotropes (Childs et al., 1987), and to perform DNA mapping using a cross-hybridization technique (Chetrit et al., 1989). 

DNA polymorphisms can be used as DNA markers. Describe the differences among RFLP, SNP, and SSR polymorphisms. How can these markers be used for DNA mapping studies ... 

Y.-H. Liu, J. Bai, X. Liang, D. M. Lubman, and P. J. Venta, Use of a nitrocellulose film substrate in matrix-assisted laser desorption/ionization mass spectrometry for DNA mapping and screening, AnaL Chem. 67, 3482-3490 (1995). 

Vianken C, Deen J, Dirix L, Stakenborg T, Dehaen W, Leen V, HoCkens J, Neely RK (2014) Super-resolution optical DNA Mapping via DNA methyltransferase-directed click chemistry. Nucleic Acids Res 42(7) c50. doi 10.1093/nar/gktl406... 

Researchers from the National Institute for Agronomical Research (INIA) have conducted projects in the field of the DNA mapping of the IP breed [17]. 

The unmasking of numerous other secondary (or noncanonical) sites that would normally avoid cleavage under stringent conditions provides a useful means for DNA mapping. The star or secondary activity can also be exploited to cleave DNA molecules containing no canonical EcoRI site. 

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