DNA-labelling

Acrylonitrile is toxic at high dose in several organs, which is possibly related to its glutathione-depleting activity. This toxicity might lead to tmnour formation by an indirect mechanism, although no data to support this are evident from the available toxicity data, such as increased cell turnover or DNA labelling. 

Figure 2. A stable DNA-branched junction. The junction shown is composed of four strands of DNA, labeled with Arabic numerals arms are labeled with Roman numerals. The 3 end of each strand is indicated by the half-arrows. Each strand is paired with two other strands to form a double helical arm. There is no homologous twofold sequence symmetry flanking the central branch point, thereby stabilizing its position.

Kessler, C., Holtke, H.-J., Seibl, R., Burg, J., and Muhlegger, K. (1990) Nonradioactive labeling and detection of nucleic acids I. A novel DNA labeling and detection system based on digoxigenimantidigoxigenin ELISA principle (digoxigenin system). Mol. Gen. Hoppe-Seyler 371, 917-927. 

The main advantage of using hybridisation indicators is that DNA-labelling procedures are avoided, but the weak point is that discrimination is not usually high enough and should be improved. 

Of all the possibilities for labeling, the first, DIG random-primed DNA labeling, is usually preferred because of its high yield. 

Russo, J. and Russo, I.H. 1978. DNA labeling index and structure of the rat mammary gland as determinant of its susceptibility to carcinogenesis. J. Natl. Cancer Inst. 61, 1451-1459. 

Joerger RD, Truby TM, Hendrickson ER, Young RM, Ebersole RC. Analyte detection with DNA-labeled antibodies and polymerase chain reaction. Clin Chem 1995 41(9) 1371—1377. 

Chiu NH, Christopoulos TK. Two-site expression immunoassay using a firefly luciferase-coding DNA label. Clin Chem 1999 45(11) 1954-1959. 

KaplanMS (1983) Proliferation of subependymal cells in the adult primate CNS differential uptake of DNA labelled precursors. J Hirnforsch 24 23-33 Kaplan MS, Bell DH (1983) Neuronal proliferation in the 9-month-old rodent— radio autographic study of granule cells in the hippocampus. Exp Brain Res 52 1-5 Kaplan MS, Bell DH (1984) Mitotic neuroblasts in the 9-day-old and 11-month-old rodent hippocampus. J Neurosci 4 1429-1441... 

Keywords DNA Labels Luminescence Probes Proteins Abbreviations... 

---