DNA-gold nanoparticle

P. Hazarika, B. Ceyhan, and C.M. Niemeyer, Sensitive detection of proteins using difunctional DNA-gold nanoparticles. Small 1, 844-848 (2005). 

C. S. Thaxton and C. A. Mirkin, DNA-gold-nanoparticle conjugates, in C. M. Niemeyer, and C. A. Mirkin, (Eds.), Nanobiotechnology Concepts, Applications and Perspectives, Wiley-VCH Verlag GmbH Co. KGaA, Weinheim, 2004, pp. 2288-2307. 

This sequence is impoitant for biotenoiism and biowaifare applications, and it has been well studied in the hterature. Hybridization of the probe and the target DNA was conducted in phosphate buffer solution with 0.3-0.5 M NaCl for half an hour at room temperature. Fluorescence studies showed that the DNA-gold nanoparticle probes are stable under high salt conditions (e g., 0.5 M NaCl). A 4-fold molar excess of a complementary strand was added and was allowed to hybridize for 30 min at room temperature. 

J.L. Chavez, W. Lyon, N. Kelley-Loughnane, M.O. Stone, Theophylline detection using an aptamer and DNA-gold nanoparticle conjugates. Biosens. Bioelectron. 26 (2010) 23-28. 

K. Lee, V.P. Drachev, J. Imdayaraj, DNA-gold nanoparticle reversible networks grown on cell surface marker sites application in diagnostics. ACS Nano 5, 2109 (2011b)... 

---