Disulfide bridges cleavage with performic acid

Disulfide bridges (crosslinkages) 65, 80 cleavage of 115-116 cleavage with performic acid 115 locating 119 in proteins 80 reduction of 115, 785 Dithioerythritol 115 Dithionite ion 779 Dithiothreitol 98,115, 822 Dityrosine linkages 81 DMSO reductase 890 DNA... 

FIGURE 5.18 Methods for cleavage of disulfide bonds in proteins, (a) Oxidative cleavage by reaction with performic acid, (b) Reductive cleavage with snlfliydryl compounds. Disulfide bridges can be broken by reduction of the S—S link with snlfliydryl agents such as 2-mercaptoethanol or dithiothreitol. Because reaction between the newly reduced —SH groups to re-establish disulfide bonds is a likelihood, S—S reduction must be followed by —SH modification (1) alkylation with iodoac-etate (ICH,COOH) or (2) modification with 3-bromopropylamine (Br— (CH,)3—NH,). 

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