Disuccinimidyl suberate,

Montesano, L., Cawley, D., and Herschman, H.R. (1982) Disuccinimidyl suberate cross-linked ricin does not inhibit cell-free protein synthesis. Biochem. Biophys. Res. Comm. 109, 7-13. 

Fig. 3. Cross-linking of l25I-labelled human GH to rabbit liver receptors. Labelled GH was incubated with receptors, cross-linked with disuccinimidyl suberate and then subjected to SDS polyacrylamide gel electrophoresis. 1. Total binding 2-4. Displacement with 1 fig unlabelled human GH, bovine GH and ovine prolactin respectively 5. l25I-human GH without receptors. Note the major GH-receptor complex of M, = 80000 (indicating a receptor protein or subunit of Af, 58000) and various other components of larger and smaller M,.

Fig. 3. Crosslinking of l25I-labelled human growth hormone to lactogenic receptors from rat liver and the Nb2 cell line. Hormone-receptor complexes were cross-linked with disuccinimidyl suberate, fractionated by SDS gel electrophoresis and detected by autoradiography. Lanes 1-4, Nb2 cells lanes 5-8, rat liver. Lines on the left indicate the positions of the hormone-receptor complex for Nb2 cells (102 kDa), rat liver (71 kDa) and uncomplexed hormone (hGH). Numbers on the right are M, values for standards (kDa). Samples were incubated with (lanes 2,4,6,8) or without (lanes 1,3,5,7) unlabelled hGH and electrophoresed under reducing (lanes 1,2,5,6) or non-reducing (lanes 3,4,7,8) conditions. From Ref. 36.

Add twice 10 p.1 of a 5 mg/ml solution of disuccinimidyl suberate (Pierce Chemicals) per ml of enzyme (30-60 s apart) with gentle stirring. 

Modification OF AFM Tips with Cross Linkers Amino-reactive AFM tip with Disuccinimidyl suberate (DSS) ... 

Disuccinimidyl suberate, a cross-linking agent, has been synthesized and used to probe the insulin-responsive D-glucose transport of the rat adipocyte. Incubation of cells with the reagent blocked methyl-a-D-glucopyranoside transport without affecting either basal hexose transport or simple diffusion as indicated by uptake in the presence of cytochalasin B. 

BS3, bis(sulfosuccinimidyl) suberate DSG, disuccinimidyl glutarate DSS, disuccinimidyl suberate GTA, glutaraldehyde HMDI, hexamelhylene diisocyanale PQQ, pyrroloquinoline quinine THPP, tris(hydroxymethyl)phosphino-propionic acid TSAT, Iris-succinimidyl aminolriacetrate. 

DTSSP, dithiobis(sulfosuccinimidylpropionate) DSP, dithiobis(succinimidylpropionate) DSS, disuccinimidyl suberate BS, Bis(sulfosuccinimidyl) suberate. 

When purely chemical cross-linking reagent disuccinimidyl suberate was used, a different conjugate of 60 kDa was found. As prior treatment of the synaptosome with proteinase K, trypsin, subtilisin, a-chymotrypsin, elastase or heat destroyed this conjugation... 

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