Dissolved oxygen with combined organic removal

Dissolved oxygen profiles with combined organic removal and nitrification... 

In a 50-mL, two-necked flask, 100 mg (0.3 mmol) of octacarbonyl dicobalt, Co2(CO)8, is dissolved in 10 mL of toluene. Then 400 mg (0.7 mmol) tppts is dissolved in 10 mL water and added to the solution. The reaction mixture is stirred for 3 h at room temperature. During the reaction the organic phase is decolorized, whereas the water phase turns brown. The mixture is transferred to a separation funnel while maintaining exclusion of oxygen. After the phases have separated, the organic phase is washed twice, in each case with 5 mL of water, and the combined aqueous phases are washed twice, in each case with 5 mL of toluene. The organic phases are discarded. The water phases are collected and the solvent is removed in a vacuum. The crude product is purified by column chromatography on Sephadex G-25. Yield 370 mg (81%) brown powder. 

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