Discriminator spectrometry

The great strength of mass spectrometry as a technique is that it can provide both the molecular weight of an analyte (the single most discriminating piece of information in structure elucidation) and information concerning the structure of the molecule involved. 

TXRF has recently also been used to discriminate between green PE garbage bags, based on Ti, Pb, Cr and Cu analysis (forensic evidence) [288], XRF provided a more effective method of discrimination when compared to IR spectrometry or DSC. 

Currently PCR and mass spectrometry are performed by two separate instruments. However, there is no reason why PCR followed by simple automated cleanup and mass spectrometry cannot be incorporated into a single integrated instrument. Essentially every configuration of the modern ESI mass spectrometer has been used successfully for the analysis of PCR products, from the highest to the lowest resolution involving. Fourier transform ion cyclotron resonance (FTICR), triple quadrupole, quadrupole-time of flight (Q-TOF), and ion trap.22-24 MS discriminates between two structurally related PCR products by MW difference. Mass accuracy is needed to differentiate the... 

D. B. Rapid discrimination between methicillin-sensitive and methicillin-resistant Staphylococcus aureus by intact cell mass spectrometry J. Med. Microbiol. 2000, 49, 295-300. 

Bernardo, K. Pakulat, N. Macht, M. Krut, O. Seifert, H. Fleer, S. Hunger, F. Kronke, M. Identification and discrimination of Staphylococcus aureus strains using matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Proteomics 2002, 2, 747-753. 

Edwards-Jones, V. Claydon, M. A. Evason, D. J. Walker, J. Fox, A. J. Gordon, D. B. Rapid discrimination between methicillin-sensitive and methicillin-resistant Staphylococcus aureus by intact cell mass spectrometry. J. Med. Microbiol. 2000, 49, 295-300. 

Vaidyanathan, S. Rowland, J. I Kell, D. B. Goodacre, R. Discrimination of aerobic endospore-forming bacteria via electrospray-ionization mass spectrometry of whole cell suspensions. Anal. Chem. 2001, 73,4134 4144. 

DISCRIMINATION AND IDENTIFICATION OF MICROORGANISMS BY PYROLYSIS MASS SPECTROMETRY FROM BURNING AMBITIONS TO COOLING EMBERS—A HISTORICAL PERSPECTIVE... 

Goodacre, R. Hiom, S. I Cheese man, S. L. Murdoch, D. Weightman, A. J. Wade, W. G. Identification and discrimination of oral asaccharolytic Eubacterium spp. using pyrolysis mass spectrometry and artificial neural networks. Curr. Microbiol. 1996,32,77-84. 

Goodacre, R. Howell, S. A. Noble, W. C. Neal, M. J. Sub-species discrimination using pyrolysis mass spectrometry and self-organising neural networks of Propionibacterium acnes isolated from normal human skin. Zbl. Bakt.-Int. J. Med. Microbiol. Virol. Parasitol. Infect. Dis. 1996,284, 501-515. 

Goodacre, R. Rooney, P. I Kell, D. B. Discrimination between methicillin-resistant and methicillin-susceptible Staphylococcus aureus using pyrolysis mass spectrometry and artificial neural networks. J. Antimicrob. Chemother. 1998, 41, 27-34. 

The most discriminating technique for proving the identity and purity of analyte peak of a chromatogram, especially for analyzing biological samples and natural products, is by using online LC-UV/MS or GC-MS/FTIR methods [15]. Alternatively, one could use a combination of TLC and MS, where direct determination on the TLC plates is made by matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) [16]. 

K. Saito, T. Mitsutani, T. Imai, Y. Matsushita and K. Fukushima, Discriminating the indistinguishable sapwood from heartwood in discolored ancient wood by direct molecular mapping of specific extractives using time of flight secondary ion mass spectrometry, Analytical Chemistry, 80, 1552 1557 (2008). 

Werner E, Heilier JF, DucruixC, EzanE, JunotC andTabet JC. 2008. Mass spectrometry for the identification of the discriminating signals from metabolomics Current status and future trends. J Chromatogr B Analyt Technol Biomed Life Sci 871(2) 143—163. 

In the determination of cadmium in seawater, for both operational reasons and ease of interpretation of the results it is necessary to separate particulate material from the sample immediately after collection. The dissolved trace metal remaining will usually exist in a variety of states of complexation and possibly also of oxidation. These may respond differently in the method, except where direct analysis is possible with a technique using high-energy excitation, such that there is no discrimination between different states of the metal. The only technique of this type with sufficiently low detection limits is carbon furnace atomic absorption spectrometry, which is subject to interference effects from the large and varying content of dissolved salts. 

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