Diphtheria Resistant mutations

Terreri AM, Rocchi P, Capucci A, et al. 1983. Induction of diphtheria toxin-resistant mutatants in human cells by halogenated compounds. J Cancer Res Clin Oncol 105 111-112. 

Gupta, R.S. Goldstein, S. (1981) Mutagen testing in the human fibroblast diphtheria toxin toxin resistance (HF Dip ) system. In de Serres, F.J. Ashby. J., eds. Evaluation of Short-Term Tests for Carcinogens. Report of the International Collaborative Program (Progress in Mutation Research, Vol. 1), Amsterdam, Elsevier, pp. 614-625... 

CIR, Chromosomal aberrations, rat liver cell line (RLi ) in vitro GIH, Gene mutation, human fibroblasts, diphtheria toxin resistance... 

Pyrolysis products were also found to induce prophage X in lysogenic E. ooli K12, strain GY5027 (30), by Inductest III, which was developed by Moreau et al, (31) (Figure 2). The genotoxic effects of pyrolysis products on cultured mammalian cells were also investigated. Trp-P-1, Trp-P-2, IQ and MelQ were found to induce diphtheria toxin-resistant mutants of Chinese hamster lung cells in the presence of S9 mix. The mutation frequencies were 33, 160, 40 and 38 per 106 survivors per Mg of Trp-P-1, Trp-P-2,... 

In the mammalian cell mutation test CHL cells are exposed to the test compound for 3 h with and without the metabolic activation system. After 7 days of culture, diphtheria toxine is added and the cells are left again to culture for another 7 days until the nmnber of diphteria toxin resistent colonies are scored. 

Gupta RS, Goldstein S. 1981. Mutagen testing in the human fibroblast diphtheria toxin resistance (HF Dipr) system. Prog Mutat Res 1 614-625. 

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