Dioxane diphosphoric acid

Phenylphosphonic dicliloride Phenyl phosphoryl dichloride Phosphate buffer (HPOOs H(PO H)xOH (NHJ,HPO, (NHJH2PO4 Dioxane diphosphoric acid HsPO ... 

Dry dicyclohexylguanidinium adenosine 5 -phosphoramidate and dry dioxane diphosphoric acid dissolved in o-chlorophenol, and stirred rapidly at 0 for 3 hrs. adenosine 5 -diphosphate. Y 83%.—Rigidly anhydrous conditions are necessary and during humid weather the dissolution is performed in a dry box. Dioxane diphosphoric acid is a convenient source of anhydrous phosphoric acid . F. e. s. R. W. Chambers, P. Shapiro, and V. Kurkov, Am. Soc. 82, 970 (1960). 

Phosphate buffer (HPO2), H(P02H)x0H (NH,)2HP0 (NHJH2PO4 Dioxane diphosphoric acid H POi... 

Phosphorylation. Chambers et al. found the reagent useful for the preparation of nucleoside diphosphoric acids (3, R —adenine, uracil, etc.). A nucleotide (I) is converted into the 5 -phosphoramidate (2) by reaction with ammonia and dicyclo-hexylcarbodiimide (DCC), and the amidate (2) is treated with dioxane diphosphate in o-chlorophenol (3 hrs..0 ). The diphosphoric acid (3) is precipitated with petroleum ether and purified as the ammonium salt. Yields are around 90%, whereas when 8.5% phosphoric add was used yields were in the order of 50-60%. However, anhydrous... 

---