1,9-Dimethylmethylene blue

Aspden RM, Larsson T, Svensson R, Heinegard D (1991) Computer-controlled mechanical testing machine for small samples of biological viscoelastic material. J Biomed Engineering 13 521-525 Farndale RW, Buttle DJ, Barrett AJ (1986) Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 83 173-177... 

In a notable paper, Lee et al. [41] examined the dimerization and inclusion complexation equilibria of six phenothiazine dyes, including thionine (TH), azure A (AZA), methylene blue (MB), toluidine blue (TB), new methylene blue (NMB) and 1,9-dimethylmethylene blue (DMMB) (Fig. 2), with CDs (a-, (>-, and y-CDs) in aqueous medium. These phenothiazine dyes presented their structural differences in the position and number of methyl substituents located on the same phenothiazine skeleton. By means of the UV-visible absorption and fluorescence spectra, the authors studied the factors which were responsible for the dimerization of phenothiazine dyes with CDs, in terms of a multiple equilibrium system (Scheme 1). In addition, the authors also determined the JCd values and the corresponding association constants of the monomers (M) and dimers (D) with the CDs [41]. 

The analysis of urinary mucopolysaccharides is best performed by a colorimetric test using dimethylmethylene blue (DMB) as described by de Jong et al. [5]. Reference values for different age groups, in use in the author s laboratory, are shown in Table D.4. 

Detector UV 525 following post-column reaction with an 18.5 xg/mL solution of 1,9-dimethylmethylene blue in water pumped at 0.5 mL/min. The column effluent mixed with the dye solution in a 3.1 p,L mixing tee and fiowed through a 28.8 cm length of 0.254 mm ID tubing to the detector. 

Biochemical assays for total DNA and Glycosaminogly-cans (GAG) contents of each sample construct (n=5) was performed. The total DNA content was done by using Hoechst 33258 dye while the total GAG content was evaluated by using the dimethylmethylene blue assay [22]. 

Skripchenko, A. and Wagner, S.J., Photoinactivation of leukocytes in erythrocyte suspensions comparison of methylene blue and dimethylmethylene blue. Transfusion, 40, 968, 2000. 

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