4-dimethylaminoazobenzene-4-sulfonyl chloride

Since the early detection of amino acids with ninhydrin, many derivatization procedures have investigated these solutes. 4-Dimethylaminoazobenzene-4 -sulfonyl chloride (DABS-C1) is performing well, but the best seems to be FMOC (9-fluorenyl chloroformate) (63). More generally, each class of reactions replaces the active hydrogens of the OH, NH, and SH groups. 

Chloride [56512-49-3]. 4 -Dimethylaminoazobenzene-4-sulfonyl chloride. Dabsyl chloride C,4H,4C1N302S M 323.802 Fluorogenic reagent for amines, amino acids and alcohols. Deep red cryst. Mp 186-188°. 

Since BAs occurring in food do not exhibit satisfactory absorbance or fluorescence in the visible or ultraviolet range, chemical derivatization, either pre- (35-37) or postcolumn (38), is usually used for their detection in HPLC. The most frequently employed reagents for precolumn derivatization are fluorescamine, aminoquinolyl-lV-hydroxysuccinimidyl carbamate (AQC) (39, 40), 9-fluorenylmethyl chloroformate (FMOC) (41-43), 4-dimethylaminoazobenzene-4 -sul-fonyl chloride (dabsylchloride, DBS) (44), N-acetylcysteine (NAC) (45,46), and 5-dimethyl-amino-1-naphthalene-1-sulfonyl chloride (dansylchloride, DNS) (47,48), phthalaldehyde (PA), and orf/to-phthaldialdehyde (OPA) (49-51), together with thiols such as 3-mercaptopropionic acid (MPA) (37) and 2-mercaptoethanol (ME) (35,49). 

Dabsyl chloride A-N,N-dimethylaminoazobenzene-4 -sulfonyl chloride) Visible 436 nm Low fmol 18-44 min precolumn Multiple products, critical concentration Stable product, good separation, high sensitivity... 

A,iV-dimethylaminoazobenzene-4 -sulfonyl — usually as the chloride, DABSYL chloride... 

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