Dimethyl sulfoxide drug testing

Drug Treatment for Inhibition of Tumor Cell Migration 1. Drug or inhibitor to be tested. Here we use cisplatin cis-diammineplatinum(ll) dichloride), stored in aliquots of 100 mM in dimethyl sulfoxide (DMSO) at -20°C for up to 4 weeks. 2. DMSO or appropriate solvent for the agent under test. 

If the cytotoxicity of the drugs being tested has not been assayed, a duplicate set of plates should be prepared without virus to check for toxic drug effects. Make up drug stock solutions as required in water, ethanol, or dimethyl sulfoxide. 

An example of a CEC analysis of an illicit drug was shown by Lurie and collaborators [134], In this study, a standard mixture of seven cannabinoids was separated, and this test mixture was then compared with concentrated hashish and marijuana extracts (Figure 5.26). The cannabinoid standards used were cannabigerol (CBG), cannabidiol (CBD), cannabinol (CBN), A-9-tetrahydrocannabinol (d9-THC), A-8-tetrahydrocannabinol (d8-THC), cannabichromene (CDB), and A-9-tetrahydrocannabinolic acid (d9-THCA-A), with dimethyl sulfoxide (DMSO) as the neutral marker. A Hypersil C18 column (3 (im dp) was used. The column inner diameter was 100 pm and had a total length of 49 cm, and the effective length was 40 cm. UV detection was conducted at 210 nm. The run buffer was 75% ACN and 25% 25 mM phosphate buffer pH 2.57. The applied voltage was 30 kV, and the column temperature was kept at 20°C. 

For the TMAH alkylaticm procedure, 100 micrograms of drug were combined with 200 microliters of a 50/50 mixture of dimethyl sulfoxide and 0.2M TMAH in methanol and 100 microliters of the appropriate alkyl halide. The mixture was allowed to stand at room temperature for 4 minutes and then heated for 4 to 6 minutes in a 60° C water bath. After the mixture was allowed to cool to room temperature, 3 milliliters of hexanes were added, vortexed briefly, and then 1 milliliter of 0.1 N potassium hydroxide was added and vortexed once again. After centrifugaticm, the top hexanes layer was transferred to a clean test tube and the hexanes layer was concentrated to approximately 200 microliters. Approximately I to 2 microliters were injected into the GC/MS instrument using the ccMiditions described above. 

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