Dimethyl suberimidate,

Dimethyl suberimidate, DMS, is a homobifunctional crosslinking agent-containing amine-reactive imidoester groups on both ends. The compound is reactive toward the s-amine groups... 

Davies, C.E., and Stark, G.R. (1970) Use of dimethyl suberimidate, a cross-linking reagent, in studying the subunit structure of oligomeric proteins. Proc. Natl. Acad. Sci. USA 66, 651. 

Uchiutni, T., Terao, K., and Ogata, K. (1980) Identification of neighboring protein pairs in rat liver 60S ribosomal subunits cross-linked with dimethyl suberimidate or dimethyl 3,3 -dithiobispropionimidate. J. Biochem. (Tokyo) 88, 1033-1044. 

PV Ramsohoye, IA Kozlov. Isoprotein composition and cross-linking of thaumatins using mushroom tyrosinase and dimethyl suberimidate. Int J Food Sci Technol 26(3) 271-282, 1991. 

Popular cross-linkers are glutaraldehyde, dimethyl adipimidate, dimethyl suberimidate, and aliphatic diamines... 

Antibodies to human immunoglobulin G (hlgG) and benzo[fl]pyrene tetraol (BPT) bound to protein A on silica beads and then cross-linked with dimethyl suberimidate hlgG and BPT, respectively [58]... 

Fig. 2 Chemical structure of glutaraldehyde (1) and dimethyl suberimidate (2) utilized for amine-amine crosslinking by Torchilin et al. [124, 125]...

Adsorption on liposome surfaces Dimethyl suberimidate- or glutaraldehyde-mediated coupling to liposome surfaces Enzyme incorporation into liposomes during formation Reaction with cellulose cyclic imidocarbonate... 

Conformationally modified RNase, derivatized with dimethyl pimelimi-date, had the highest fluorohydrolase activity toward DFP however, the highest activity toward PMSF was obtained for dimethyl suberimidate crosslinked protein. The best overall activity for both substrates was obtained with the pimelimidate (Cs) crosslinked modified RNase. A plot of activity towards PMSF versus pH, after chromatography on G-15 Sephadex, shows that the optimum pH is approximately 7.5 (Figure 2). The values of... 

The location of the binding site of cyt c was investigated by several different approaches (17). In one, crosslinking agents (e.g., dithiobis-propionimidate, dimethyl suberimidate were used to link cyt c and cyt C2... 

Dimethyl suberimidate (DMS) was obtained from Pierce Chemical Co., hexamethylene diisocyanate (DIG) was obtained from Aldrich Chemical Co. Published procedures were employed for preparation of spinach chloroplasts (Kamienietzky, Nelson 1975) H -ATPase complexes (Nelson et al. 1980 Rott, Nelson 1981) and antibodies Nelson et al. 1973) for... 

Since subunit A did not bind to the membranes in the end of the equilibrium binding assay, obviously the dissociation of the crotoxin complex at some point is an important step in the action of the toxin. Hendon and Tu (28) converted crotoxin complex into a non-dis-sociable conjugate by cross-linking the two subunits with a bifunctional cross-linker, dimethyl suberimidate. This conversion abolished the lethality of crotoxin but did not significantly reduce its PLA2 activity. When we used the non-dissociable crotoxin in binding... 

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