Dimethyl adipimidate,

Hartman, F.C., and Wold, F. (1967) Cross-linking of bovine pancreatic ribonuclease A with dimethyl adipimidate. Biochemistry 6, 2439-2448. 

Mentzer Jr., W.C., Lewis, S., Pennathur-Das, R., Halpin, R., Cerrone, K.L., Lubin, B., and Kenyon, G.L. (1982) Formation of 5-carbomethoxyvaleramidine during hydrolysis of the protein cross-linking agent dimethyl adipimidate./. Brotein Chem. 1, 141-155. 

Pennathur-Das, R., Heath, R., Mentzer, W.C., and Lubin, B. (1982) Modification of hemoglobin s with dimethyl adipimidate. Contribution of individual reacted subunits to changes in properties. Biochim. Biophys. Acta 704, 389-397. 

Waterman, M.R., Yanaoka, K., Chuang, A.H., and Cottam, G.L. (1975) Anti-sickling nature of dimethyl adipimidate. Biochem. Biophys. Res. Comm. 63, 580-587. 

Dimethyl adipimidate dihydrochloride [14620-72-5] M 245.1, m 218-220 , 222-224 . If the salt smells of HCl then wash with MeOH and dry Et2O (1 3) under N2 until the HCl is completely removed. Recryst from MeOH-Et2O (it is very important that the solvents are super dry) [Hartman and Wold Biochemistry 6 2439 1967 McElvain and Shroeder JACS 71 40 1949. ... 

Hartman and Wold (212) have used a bifunctional imido ester, dimethyl adipimidate, as a cross-linking reagent for an enzyme sample containing phosphate ion to protect Lys 41. The monomeric component of the reaction mixture was apparently itself a mixture of species which was not further fractionated. The total fraction had slightly reduced RNA activity but the same Vm and a decreased Km for the activity... 

Popular cross-linkers are glutaraldehyde, dimethyl adipimidate, dimethyl suberimidate, and aliphatic diamines... 

Areas close to binding domains that hold together layers of three subunits react preferentially with the cross-linking reagent dimethyl-adipimidate to form trimers, which are recognized by their electrophoretic mobility on polyacrylamide in SDS (141)- It has not yet been possible to separate active trimers. 

The inner surface of nylon tubing has been activated with dimethylsulphate and then treated with L-lysine. After activation of the derivatized surface with glutaraldehyde or dimethyl adipimidate, neuraminidase was covalently linked to the surface of the tubing. The stability of the active immobilized enzyme was investigated and its potential application for release of neuraminic acid from surfaces of leukaemic AKR mouse thymus and spleen lymphocytes evaluated. 

Thus (77) 47) is a dimeric derivative of N-acetyl-muramyl-L-alanyl-D-isoglutamine-L-lysine [MDP-Lys, (63)], in which the e-amine functions of each monomeric glycotripeptide unit are linked by an adipimidyl residue, the coupling being achieved by means of dimethyl adipimidate 23. 48). 

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