Diisopropyl fluorophosphate exposure

Prendegast, M.A., Terry, A.V., Buccafusco, J.J. (1997). Chronic, low-level exposure to diisopropyl fluorophosphate causes protracted impairment of spatial navigation learning. Psychopharmacology 129 183-91. 

Aeylpeptide hydrolase is a member of the serine hydrolase family. It deaeetylates the acetylated N-terminus of polypeptides. Rat brain aeylpeptide hydrolase was inhibited 93% at a dose of diehlorvos (4mg/kg, i.p.) which inhibited aeetyleholinesterase only 47%. The in vitro sensitivity of aeylpeptide hydrolase to chlorpyrifosmethyl oxon, diehlorvos, and diisopropyl fluorophosphate (IC50) was 6-10 times greater than that of acetylcholinesterase (Riehards et al, 2000). Aeylpeptide hydrolase is also found in human erythroeytes where it could potentially serve as a biomarker for low dose exposure to OP in humans, though human eases of OP exposure have not yet been tested for OP-modified acylpeptide hydrolase. 

In a similar series of experiments, our laboratory demonstrated that topical exposure to a nerve agent stimulant, diisopropyl fluorophosphate (DFP), more than doubled permethrin absorption (Baynes etal., 2002). This significant effect on... 

Because of its high concentration in human plasma, BChE is also used as a biomarker for detection of exposure to OP pesticides and nerve agents. Hence, the sensor was likewise evaluated for detection of BChE-OP biomarkers. The detection method is the same as the protocol described previously in section 4.1 however, the chemical nerve agent (stracturally similar to Sarin) diisopropyl fluorophosphate (DFP) was chosen as a model OP in this studies. 

There have been descriptions of the acute effects in humans that follow high-dose exposure (>LDso) to nerve agents soman, sarin, and VX (Inoue, 1995 Nozaki et al., 1995 Nakajima et al., 1997). The similar cluster of behavioral symptoms (anxiety, psychomotor depression, intellectual impairment, and sleep disturbance) was observed in the immediate period after resolution of the acute signs of intoxication and then slowly faded with time, somehmes taking months to be fully resolved. The CNS symptoms noted after short-term exposure of humans to diisopropyl fluorophosphate (DFP) were... 

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