Diimide hydrochloride

Ethyl diazoacetate, 228-230,325 Ethyl diethoxyacetate, 230 Ethyldiisopropylamine, 230,368 Ethyl (3,0-dimethylacrylate, 249 l-Ethyl-3-(3 -dunethylaminopropyl)carbo-diimide hydrochloride, 26 Ethyl l,3-dithiane-2-carboxylate, 230-231 Ethylene, 11 Ethylene carbonate, 41 Ethylenediamine, 231,488 Ethylenediaminetetraacetic acid, 204 Ethylenediaminetetraacetic add disodium salt, 321... 

Coupung, dehydrative Bis-o-phenylene pyrophosphite. N,N -Carbonyldiimidazole. N,N -Carbonyl-J-triazine. l-Cyclohexyl-3-(2-morpholinomethyl)-carbodiimide. 1,1-Di-chlorodiethyl ether. Dicyclohexylcarbodiimide. Diethyl chlorophosphonate. Diethyl-cyanamide. Diethyl ethylenepyrophosphite. N-(3-Dimethylaminopropyl)-N -ethylcarbo-diimide hydrochloride. Diphenylketene p-tolylamine. Ethoxyacetylene. l-Ethyl-3(3 -di-methylaminopropyllcarbodiimide hydrochloride. Ethylene chlorophosphite. N-Ethyl-S-phenyliioxazolium-3 -sulfonate. N-Hydropyridine. N-Hydroxyphthalimide. N-Hydroxy-piperidine, N-Hydroxysuccinimide. Phenylphosphorodi-d-imidazolate). 

EDCI l-ethyl-3-[3-(dimethyl-amino)propyl]carbo-diimide hydrochloride... 

Sample preparation 10 mg Etodolac + 10 mg l-(3-dimethylaminopropyl)-3-ethylcarbo-diimide hydrochloride -I- 2 drops 3,5-dimethylaniline -t- 1.5 mL dichloromethane, mix. 

An N-protected amino aldehyde can be prepared by Swem oxidation of an N-pro-tected amino alcohol in the presence of an oxidizing agent comprising DMSO and a water-soluble carbodiimide derivative as l-ethyl-3-(3-dimethylaminopropyl)carbo-diimide hydrochloride (EDC) in an organic solvent. The N-protected amino alcohol can be derived from an L-, D-, or L-/D-amino acid, but usually is the N-protected-(S)-a-amino alcohol derived from an N-protected L-amino acid. 

Chloroacetylhydrazide hydrochloride, ClCHjCONHNHj-HCl. Mol. wt. 145.00, m.p. 150° dec. The reagent is prepared by reaction of the activated p-nitrophenyl ester (1) with hydrazine. It is used for the generation of diimide, which see. 

According to a preliminary report, diimide can be generated at 0° by the action of sodium hydroxide on chloroacetylhydrazide hydrochloride (which see). Another precursor is hydroxylamine-O-sulfonic acid (which see). Azobenzene has been reduced quantitatively to hydrazobenzene and tdlyl alcohol to propanol (70%). ClCHjCONHNHi HCl + 2 NaOH ----------> CH2=C=0 + HjO + HN=NH + 2 NaCff... 

Diimide precursors Chloroacetylhydrazide hydrochloride. Hydrazine. Hydroxylamine-O-sulfonic acid. Potassium azodicarboxylate. o-Diketone adduct Triethyl phosphite. 

Benzyl(methyl)sulfur diimide reacts with sodium dicyanamide in the presence of three equivalents of hydrochloric acid in diethyl ether to give the corresponding 1 -methyl-1/4,2,4,6-thiatri-azine-3,5-diamine hydrochloride (10) with elimination of benzyl chloride.40... 

Phthaloylleucylvaline ethyl ester 710 DL-Valine ethyl ester hydrochloride (1.82 g, 10 mmoles) in dry tetrahydrofuran (20 ml) is treated at 0° with triethylamine (1.38 ml). The triethylamine hydrochloride is filtered off after an hour and washed well with tetrahydrofuran. Phthaloyl-leucine (2.57 g, 10 mmoles) is dissolved in the filtrate, and then at —8° dicyclohexylcarbo-diimide (2.26 g, 11 mmoles). The mixture is left for 2 h at — 5° and then at room temperature overnight. The separated 1,3-dicyclohexylurea is filtered off and the filtrate is evaporated in a vacuum, leaving a syrup which is dissolved in ethyl acetate (25 ml) and washed with 2n-hydrochloric acid (25 ml) and water (two 25-ml portions). The solution is dried over sodium sulfate, filtered, and evaporated in a vacuum, the residue (3.5 g, 90%) solidifying to a glass on storage this product is very soluble in alcohols, chloroform, ethyl acetate, pyridine, or dimethylformamide, moderately soluble in benzene or toluene, but insoluble in ether, light petroleum, or water. 

Potassium azodicarboxylate and triethylamine hydrochloride are placed in flask C, and this section of the apparatus is connected to the vacuum line and evacuated. The breakseal is then broken with the magnet, and the evacuated apparatus is removed from the vacuum line and tilted to pour the phenyldiazene solution onto the diimide precursors. Phenylhydrazine formation is detected by measuring the ultraviolet spectrum of the solution via the quartz cell. Over the course of a few hours, the spectrum changes from that of phenyldiazene to that of phenylhydrazine. 

Ferritin and bovine serum albumin (BSA) proteins can also chemically bond to nitrogen-doped MWNTs (CNx-MWNTs) through a two-step process of diimide-activated amidation. Firstly, carboxylated CNx-MWNTs were activated by N-ethyl-N -(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC), forming a stable active ester in the presence of N-hydroxysuccinimide (NHS). Secondly, the active ester was reacted with the amine groups on the proteins of ferritin or BSA, fornting an antide bond between the CNx-MWNTs and proteins. This two-step process avoids the intermolecular conjugation of proteins, and guarantees the uniform attachment of proteins on NTs (Scheme 12.3) [44]. 

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