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Differential gene expression, monitoring

Protein expression profiling (protein differential display) using microarrays is considered an important new tool for proteomic discovery. It is similar in concept and approach to the gene expression microarray for mRNA profiling. Sreekumar and Chinnaiyan (2002) describe a general approach for using the microarray to monitor protein expression in cancer and normal tissues. Here are the steps ... [Pg.22]

In a recent study (Fridley et al., 2010), spinner flasks and rotary bioreactors were compared in terms of hematopoietic differentiation efhcacy and progenitor cell profile were examined. cDNA microarrays were used to monitor mouse ESC gene expression profile during differentiation under dynamic conditions and it was observed that cells from all three germ layers were generated during bioreactor cultures, with distinct profiles in each bioreactor, in particular for hematopoietic differentiation. [Pg.773]

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