DHA-Utilizing Enzymes

Example of multistep chemo-enzymatic synthesis of pipecolic acid derivatives (60) and homoiminocyclitols (61) by two consecutive enzymatic aldol addition reactions (a) i-serine hydroxy-methyltransferase from Streptococcus thermophilus (lSHMTj ) (b) Cbz-OSu CHjCN/aqueous HCI (c) Cbz-OSu MeOH/SO CIj CaCI, NaBH, EtOH/THF CHjCN/aqueous HCI (d) o-threonine aldolase from Achromobacterxylosoxidans (oThrA j (e) FucA F131A (f) RhuA wild-type (g) acid phosphatase from potato type II and (h) H Pd/C. [Pg.282]

Aminocyclitol (e.g., 63) synthesis from nitroaldehydes (e.g., 62). Aldol addition of DHAP to nitroaldehyde 62 cataiyzed by DHAP-dependent aldolases followed by an ensuing spontaneous intramolecular Henry reaction, (a) DHAP-dependent aldolase and (b) Dephosphorylation by acid phosphatase and subsequent catalytic reduction of the nitro group. [Pg.283]

Addtion of GO FSAA129G FSA A129T, FSA L107Y/A129G/A165G [Pg.283]

Modeled active site structure of wild-type FSA in complex with D-fructose-6-phosphate (F6P) enamine intermediate. Position and changes in the amino acid residues to alter the donor and acceptor substrate tolerance. [Pg.283]

10 RECENT ADVANCES IN ENZYME-CATALYZED ALDOL ADDITION REACTIONS [Pg.284]

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