Dextrane analogs, synthesis

Considerable interest has been expressed in benzoylated derivatives of 2-diethylaminoethylated polysaccharides, the acylated derivative being produced by the action of benzoyl chloride on, for example, 0-(2-di-ethylaminoethyl)cellulose. Naphthoylated and benzoylated, naphtho-ylated 0-(2-diethylaminoethyl) celluloses may be similarly produced, and these substitutions of the free hydroxyl groups in the 0-(2-diethyl-aminoethyl)celluose increase the non-ionic attractions between the polysaccharide and polynucleotides. This phenomenon has been exploited in the synthesis and separation of soluble ribonucleic acids. " Some difficulties have been experienced in producing sizable quantities of benzoylated 0-(2-diethylaminoethyl)cellulose, but the analogous preparation of benzoylated O-(2-diethylaminoethyl) cross-linked dextran was more successful, the product having properties similar to those of the corresponding cellulose derivative. 

In Uppsala, J. Porath and P. Flodin developed an extremely important method for the separation of peptides and proteins gel permeation chromatography with crosslinked dextran (Sephadex). One of Porath s former associates, Ulf Ragnarsson contributed with innovative modifications to the solid phase method of peptide synthesis and in more recent years introduced the principle of complete protection of the amine function by diacylation. Another Porath student, Gunnar Lindeberg, explored the properties of vasopressin analogs. 

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