Dexamethasone liquid chromatographic methods

Qin, Y Liang, D. Zeng, J. Mao, W. [Determination of hydrocortisone and methylprednisolone in plasma by reversed-phase HPLC]. Hua Hsi I Ko Ta Hsueh Hsueh Poo, 1991, 22, 270-273 Shalaby, A. Shalyahan, M. Improved high performance liquid chromatographic method for the determination of some corticosteroids. J.Liq.Chromatogr., 1991, 14, 1267—1274 [formulations ointment lotion tablets iiyections simultaneous dexamethasone, prednisolone]... 

Over the past 10 years, liquid chromatography coupled with ultraviolet detection appears to have become the method of choice for the determination of corticosteroids, offering tlie analyst both satisfactory selectivity and sensitivity. Both reversed-phase (544-547) and normal-phase (548) chromatography have been applied to the determination of dexamethasone in plasma, coupled with ultraviolet (UV) detection generally at 254 nm, and in bovine tissues (528, 535). A series of both reversed- and normal phase LC systems have also been used for the simultaneous determination of dexamethasone and other steroids. Two different liquid chromatographic separations have been described for the isolation and simultaneous separation of steroids in serum (549). 

L. L. Ng, Reverse phase liquid chromatographic determination of dexamethasone acetate in cortisone acetate in bulk drug substances and dosage forms Method development, J. Assoc. Off. Anal. Chem., 70 829(1987). 

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