Development of a Generalized Kinetic Model for One-Substrate Reactions Under Inhibition

2 Development of a Generalized Kinetic Model for One-Substrate Reactions Under Inhibition 

Inhibition by products and/or substrates is quite relevant so that a generalized equation will be derived considering most of the situations of inhibition kinetics for one substrate reactions (Siimer 1978). The following reaction scheme will be considered  

Determination of reaction kinetics for any mechanism involving one-substrate (or two substrates when one is in large excess) can be easily derived from Eq. 3.41 and the above reaction scheme by considering those kinetic constants that have finite values as indicated in Table 3.2. 

As an example, in the case of the enzyme (3-galactosidase, that catalyzes the hydrolysis of lactose into glucose and galactose, which is inhibited competitively 

In the case of penicillin acylase, that catalyzes the hydrolysis of penicillin G into 6-aminopenicillanic acid and phenylacetic acid, which is inhibited by 6-aminopenicillanic acid non-competitively and by phenylacetic acid competitively, from Table 3.2 only K, Ki, K2, K2, and kcat have finite values. Then, from Eq. 3.41  

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