Deuterium exchange methods

Ohashi, N., Furuuchi, S., and Yoshikawa, M. (1998). Usefulness of the hydrogen-deuterium exchange method in the study of drug metabolism using liquid chromatography-tandem mass spectrometry. J. Pharm. Biomed Anal. 18 325-334. 

The deuterium exchange method was also used to determine the average value of the silanol number as a function of dehydroxylation temperature (473 - 1373 K in vacuo). The experimental results are shown in figure 4.1. 

The components of the silanol number (free and bridged hydroxyls) were determined by the deuterium exchange method and infrared spectroscopic measurements.36... 

On the experimental front, the structure of PrPSc derived from brain tissue of scrapie-infected transgenic mice expressing GPI-free prion protein was recently examined by the hydrogen/deuterium exchange method [105], In contrast to the models described above, these data indicate that the PrPc >PrPSc conversion involves major refolding of the entire region C-terminal to residue 80-90, and that this region in PrPSc consists of a network of (3-strands and relatively short turns, with no native a-helices present. 

Labeled amino acides. [DsJ-L-Tryptophan and [DsJ-tryptamin were prepared by the deuterium-exchange method as previously described. [ CsJ-L-Isoleucine and [ N2]-L-arginine were obtained from Cambridge Isotope Laboratories and Spectra Stable Isotopes, respectively. 

The Kiselev-Zhuravlev constant aoH value of 4.6 was obtained with a deuterium-exchange method that distinguished between surface and bulk OH and with a mass spectrometric thermal analysis (MTA) method in conjunction with temperature-programmed desorption (TPD) (66). 

Gabelova and Kobyakov (1965) have used deuterium exchange methods to investigate the simple muscle contraction system of actomyosin (myosin B) and its property of syneresis during interaction with adenosine triphosphate (ATP). The purpose of the work was to look for a molecular basis of muscle contraction and to see if there is any change in protein structure in a contracted muscle. The initial spectrum of actomyosin (the control) and that of actomyosin after syneresis (i.e., with ATP present) showed no essentia) difference. However, the usual spectral changes produced by partial deuteration of proteins appeared in the spectrum of actomyosin and of actomyosin plus ATP. Instead of a broad band near 3300 cm a much... 

M. J. Chalmers, S. A. Busby, B. D. Pasctil, M. R. Southern and P. R. Griffin. (2007) A Two-Stage Differential Hydrogen Deuterium Exchange Method for the Rapid Characterization of Protein/ligand Interactions, Journal of Biomolecular Techniques, 18 (4), 194—204. 

The hydrogen-deuterium exchange method can be used to study secondary, tertiary, and even quaternary structures. Amide hydrogens in the hydrophobic core of the protein or at the interface of attached subunits are less accessible for the exchange reaction. Studying the kinetics of the exchange can reveal unfolding... 

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