Determination of water soluble carbohydrate by autoanalysis

Method 7.14. Determination of water soluble carbohydrate by autoanalysis 

See the discussion in Chapter 4 Nitrate and water soluble carbohydrate. Note the extract obtained for determining nitrate in plants, which is also in saturated benzoic acid, may be used for the water soluble carbohydrate determination if diluted x2. 

Calculation. Draw a baseline on the chart under all the sample peaks by connecting the baseline from aspirating wash at the start, between trays and at the end. Read the concentration of the sample solutions by comparing the peak heights of the samples with the standards using a chart reader (see Chapter 1, Chart reader ). Divide the concentration in pg mb of soluble carbohydrate in the sample solution by 10 to get the % water soluble carbohydrate in the freeze-dried sample. Multiply by 100/(100 - % moisture) to give the percentage water soluble carbohydrate in the sample DM. 

---