Detection of Clostridium botulinum

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Recently, a novel approach for the detection of Clostridium botulinum neurotoxins was developed (Ogert et al., 1992). This technique involved a two-step sandwich immunoassay utilizing an evanescent wave of light to excite fiuorescently labeled antibodies bound to type A toxin captured on the surface of antibody immobilized fibers. Despite the many... 

Figure 3.Two-step sandwich immunoassay used in the detection of Clostridium botulinum neurotoxins.

Table 1. Available Techniques for the Detection of Clostridium botulinum neurotoxins...

Furthermore, research efforts described herein, have resulted in at least a ten-fold improvement in the detection of Clostridium botulinum neurotoxin using a fiberoptic immunosensor. Additionally, the increased sensitivity of the assay by using affinity purified polyclonal antibodies as both the immobilized capture antibodies and the fluorescently labeled antibodies suggests that the investigation into additional assay systems may be worthwhile. 

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