Desorption 7-globulin

Neither the presence of heparin nor that of EDTA in solution inhibited desorption by intact plasma (Table III). On the other hand, when a preformed globulin film was first exposed to protamine sulfate, though this exposure affected film thickness very little (Table IV), normal intact plasma could no longer remove matter but only deposit it (Table IV, Exp. No. 2). 

Elwing, H. Askendal A. Lundstrom I., Desorption of fibrinogen and gamma-globulin from solid surfaces induced by a nonionic detergent, J. Colloid Interface Sci. 1989, 128, 296-300... 

The exchange of proteins (albumin, globulin, and fibrinogen) and the time course of adsorption-desorption from polymers have been studied by... 

Interfacial protein fluorescence is an in situ method that can provide real time data with a resolution of 0.1 s. This technique is a major advantage in that the protein adsorption-desorption dynamics may be determined without resorting to sample manipulation prior to analysis. Figure 9 illustrates adsorption-desorption dynamics for both BSA and 7-globulin at bulk equimolar concentrations of 6.671xM/L. The 7-globulin required 40 min to reach... 

Figure 9. Adsorption-desorption dynamics for equimolar concentrations of BSA and y-globulin on hydrophilic quartz at 37°C.

Figure 11. Desorption dynamics for 6.67 pM/L BSA (o) and y-globulin (9) on hydrophilic quartz at 37°C.

The question remains whether the denatured protein molecule regains its native structure on desorption. Soderquist and Walton extensively studied the structure of three plasma proteins—albumin, y-globulin and fibrinogen—on desorption from different co-polypeptide (amino acid copolymers) and silicone surfaces under different pH and ionic strength conditions [88]. Circular dichroism (CD) analysis of the surface desorbed proteins showed a marked decrease in the a-helical content for albiunin and fibrinogen, whereas y-globulin lost most of its P-sheet structure. The reduction... 

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