Deoxycytidine kinase, properties

Coleman, C.N. Stoller, R.G. Drake, J.C. Chabner, B.A. Deoxycytidine kinase properties of the enzyme from human leukemic granulocytes. Blood, 46, 791-803 (1975)... 

Nucleoside kinase inhibitors hold promise for the development of anticancer drugs by inhibition of the salvage pathway. Although previous attempts to develop these types of inhibitors into drugs have failed, the majority of these efforts focused on nucleoside analogs with poor physicochemical properties. Several non-nucleoside inhibitors have been reported for adenosine kinase and deoxycytidine kinase, with promising initial properties. It is possible that future efforts may result in the identification of non-nucleoside inhibitors that possess an appropriate efficacy-side effect profile. 

S Additional information <3, 7> (<3> enzyme exists in different conformational states with different substrate kinetic properties [9] <3> presumably one common nucleoside acceptor site [15] <3> purine deoxynucleo-side activity inseparably associated with deoxycytidine kinase protein [16] <3> several isozymes cytosolic deoxycytidine kinase I and II, plus mitochondrial isozyme [10] <3> multisubstrate enzyme, that also phos-phorylates purine deoxyribonucleotides [9] <7> enzyme has two separate active sites for deoxycytidine and deoxyadenosine activity [22] <3> reacts with both enantiomers of -deoxycytidine, -deoxyguanidine, -deoxyadenosine, and a-D-deoxycytidine is also substrate [31] <3> reacts with both enantiomers of jS-deoxyadenosine, j3-arabinofuranosyl-adenine and jd-deoxyguanine ]34] <3> remarkably relaxed enantioselectivity with respect to cytidine derivatives in p configuration [36] <3> lack of enantioselectivity for D- and L-analogues of cytidine and adenosine [43]) [9, 10, 15, 16, 22, 31, 34, 43]... 

Kessel, D. Properties of deoxycytidine kinase partially purified from L1210 cells. J. Biol. Chem., 243, 4739-4744 (1968)... 

Kozai, Y. Sonoda, S. Kobayashi, S. Sugino, Y. Metabolism in deoxyribo-nucleotides. IV. Purification and properties of deoxycytidine kinase of calf thymus. J. Biochem., 71, 485-496 (1972)... 

Johansson, M. Karlsson, A. Differences in kinetic properties of pure recombinant human and mouse deoxycytidine kinase. Biochem. Pharmacol., 50, 163-168 (1995)... 

Shafiee, M. Gosselin, G. Imbach, J.-L. Divita, G. Eriksson, S. Maury, G. Study of human deoxycytidine kinase binding properties using intrinsic fluorescence or new fluorescent ligands. Eur.J.Med.Chem., 34, 423-431 (1999)... 

S ATP -I- deoxycytidine <1, 3, 4, 8> (<3> deoxycytidine kinase is a multisubstrate enzyme that also phosphorylates deoxyadenosine, it exists in different conformational states with different substrate kinetic properties... 

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