Deoxycorticosterone binding

DOC Deoxycorticosterone TEBG Testosterone-estrogen-binding globulin... 

Human corticosteroid binding globulin (CBG) has been the target for photoaffinity labeling by the carbene precursor, [6,7-3H]-21-diazo-21-deoxycorticosterone (Compound V)... 

Figure 8-39. Competition of various steroids with radioactive deoxycorticosterone (DOC) for binding to DOC-specific antiserum. These data were generated by standard radioimmunoassay procedures. (Courtesy of New England Nuclear Corp., Worcester, Mass.)...

Mitochondrial CYP11B2 (also known as CYPllp or steroid lip-hydroxylase) is involved in the synthesis of aldosterone. Aldosterone affects the conservation of sodium, the secretion of potassium, water retention, and blood pressure. CYPl 1B2 has been shown to catalyze the terminal regio- and stereospecific hydroxylation of deoxycorticosterone to aldosterone however, the exact mechanism of this process was not solved. Rapid-quenching experiments with the membrane-bound bovine CYP11B2 incorporated into liposome membranes have demonstrated that aldosterone is produced via corticosterone and not via 18-hydroxydeoxycorticosterone. Moreover, a kinetic analysis suggested a successive mechanism of aldosterone production from corticosterone, which did not dissociate from the binding site of the enzyme [34] (Scheme 5.5). 

Deoxycorticosterone hemisuccinate (17) Purification of progesterone-binding globulin by affinity chromatography 153... 

---