Delvotest SP

Results showed a total of 2.8% of the samples (n 2972) to be inhibitor positive by the Delvotest SP test further examination identified 1.7% as -lactam antibiotics, and 1.1 % as sulfonamides and dapsone. The percentage of chloramphenicol suspicious samples determined by the Charm II test was amazingly high however, tests for confirmation were not available and contamination of the samples by residues of the chloramphenicol-based preservative azidiol could not be excluded with certainty. Low concentrations of streptomycins were also detected in 5.7% of the samples (n 1221), but the MRL was not exceeded. Macrolide and tetracycline residues were not found in significant levels. Model trials with commercially applied yoghurt cultures confirmed how important the compliance to MRLs can be to dairy industry compared to antibiotic-free milk, a pH of 5.0 was reached with a delay of 15 min in the case of contamination with cloxacillin 30 min in the case of penicillin, spiramycin, and tylosin and 45 min in the case of oxytetracycline contamination. 

DelvoTest SP. This test has enhanced sensitivity to the sulfonamide class, as well as the ability to detect... 

Marker Residue) Milk (p.g/kg) Eclipse 100 DelvoTest SP DelvoTest SP-NT CMT Blue Yellow II Cowside II... 

More recently, the DelvoTest Accelerator system for the combined incubation and measurement of the DelvoTest SP-NT ampoules and multi-plates (Section 5.2.3) has been launched by DSM Food Specialties. The test ampoules or multi-plates are placed directly onto the scanner glass top plate the glass plate is heated to 63°C to perform the incubation step. The Accelerator system can run up to 100 individual ampoules or four 96-well multi-plates (potential for 384 samples) in parallel. A scanner measurement is taken every minute until the test end-point is reached, which is determined automatically via the software control system. The incubation time is shorter than the conventional system, at around 100 min. Once the end-point is reached, the test is automatically terminated and the results stored for subsequent assessment, meaning that the tests can be performed out of the normal working hours. 

Figure 5.2 Radar plot showing the DelvoTest (SP-NT ampoules and multi-plate formats) lowest limit of detection for 10 antimicrobial compounds in bovine milk expressed as a fraction of the current EU MRLs.

The DelvoTest Accelerator system in combination with the DelvoTest SP-NT ampoules and multi-plates were validated in milk in accordance to the requirements of the International Dairy Federation. The estimated detection limits of the DelvoTest SP-NT ampoules and multi-plates are expressed as a fraction of the current EU MRLs (Fig. 5.2). With the exceptions of oxytetracycline and neomycin, all the analytes included in the study were detected at or below the EU MRL by both test formats. This finding reflects the degree of susceptibility of the microorganism employed in the test to the range of antimicrobial compounds tested. 

Gibbons-Burgener et al. " evaluated the performance of the rapid SNAPBL, DelvoTest SP, and Penzyme milk test-kits for testing raw milk samples from individual cows. 

Stead SL, Ashwin H, Richmond SF, Sharman M, Langeveld PC, Barendse JP, Stark J, Keely BJ, Evaluation and validation according to international standards of the Delvotest SP-NT screening assay for antimicrobial drugs in milk, Int-natl. Dairy J. 2008 18 3-11. 

Le Breton MH, Savoy-Perroud MC, Diserens JM, Vahdation and comparison of the Copan milk test and Delvotest SP-NT for the detection of antimicrobials in milk. Anal. Chim. Acta 2007 586(l-2) 280-283. 

Delvotest P/SP/ Direct assay B. ste rothermophilus 2.5 h at 64 C into Bromocresol Penicillineise 130 ... 

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