Definition of chromatography

Give a general definition of chromatography that would apply to all types and configurations. (To say that it is a separation technique is important but not sufficient.)... 

Chromatography is the separation of a mixture of compounds into its individual components based on their relative interactions with an inert matrix. However, chromatography is more than a simple technique, it is an important part of science encompassing chemistry, physical chemistry, chemical engineering, biochemistry and cutting through different fields. It is worth to be mentioned here that the lUPAC definition of chromatography is "separation of sample components after their distribution between two phases". 

Using the definition above (or any other definition of chromatography), one can tabulate numerous variations of the technique (see Figure 1.1). Our specific concern is the technique of gas chromatography. For this technique we have available different... 

The usual definition of chromatography as a method of separation does not imply that a detector is involved. In TLC and PC, detectors are not required for qualitative analysis, and consequently, these simple techniques are used primarily for qualitative screening. The opposite is true for the column methods, and one normally assumes that a chromatograph includes a detector. This section deals with the general aspects of detectors like those used in GC and LC column chromatographs. 

Definition of chromatography, lUPAC (1993) Chromatography is a physical method of separation in which the components to be separated are distributed between two phases, one of which is stationary while the other moves in a definite direction. ... 

Salt Effects. The definition of a capacity factor k in hydrophobic interaction chromatography is analogous to the distribution coefficient, in gel permeation chromatography ... 

These are the definitions of the two characteristic dissociation constants normally expressed in terms of p K. When three dissociating groups are present in a molecule there are three piC values, ie, pfC, P 3- knowledge of these piC values is important in the separation or isolation of each amino acid by ion-exchange chromatography. 

Finally, an officially updated definition of the retardation factor, R, issued by lUPAC is important to the whole field of planar chromatography (the linear and the nonlinear TLC mode included). The importance of such a definition has two reasons. First, it is promoted by the growing access of planar chromatography users for densitometric evaluation of their chromatograms and second, by the vagueness of the present definition in the case of skewed concentration profiles with the samples developed under mass overload conditions. 

When you start working across the Internet, the chromatography data system becomes an open system and the FDA rule requires controls. Using FDA s definition of electronic records, the laboratory chromatography data system generates electronic records. Based upon the definition, laboratories will need to consider more than just the raw data tiles. One must also include the method tiles, mn sequence tiles, and the integration parameters used for the data analysis. The need for a comprehensive audit trail is a critical component of the FDA regulations. The audit trail is an electronic record and is subject to the same controls. 

The general steps in developing an acceptable analytical method in liquid chromatography are summarized in Figure 4.26. Method development starts with a clear definition of the needs of the analysis. How many detectable components are present in the ample Are all peaks equally relevant In the first case all peaks must be resolved and the difficulty of providing the desired result will increase with the number of components in the sample. 

Complete the following definition of liquid chromatography by filling in the blanks. For each space, choose a word from the groups given below ... 

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