DEDAS

However, several important studies have shown that intravenous thrombolysis may be beneficial more than 3 hours after stroke onset, provided that only patients with a significant diffusion-perfusion mismatch are treated. In one such smdy, Ribo et al. found that patients with a significant diffusion-perfusion mismatch could be treated safely and effectively in the 3-6-hour time period. In phase II of the desmo-teplase in acute stroke (DIAS) trial, patients with diffusion-perfusion mismatch were treated with desmoteplase up to 9 hours after stroke onset, and showed better outcomes than patients given placebo, with only a minimal incidence of symptomatic hemorrhage. Similar success was achieved in the same time window by the dose escalation study of desmoteplase in acute ischemic stroke (DEDAS). ... 

Eurlan AJ, Eyding D, Albers GW, Al-Rawi Y, Lees KR, Rowley HA, Sachara C, Soehngen M, Warach S, Hacke W, Investigators D. Dose Escalation of Desmoteplase for Acute Ischemic Stroke (DEDAS) evidence of safety and efficacy 3 to 9 hours after stroke onset. Stroke 2006 37 1227-1231. 

Transposon integration mutagenesis can be used to allow the selection of metalloid-resistant mutants with enhanced MICs.147 Ledgham et al.148 found a transposon-disrupted gene in a mutant - with a three-fold increase in Se-resistance - that encoded a protein from a member of the DedA family of membrane proteins that was implicated in the uptake of selenite through cell membranes. This is a reasonable hypothesis for how metalloid resistance occurs in some microorganisms. 

Gogarten JP, Kiblak H, Dittrich P, Taiz L, Bowman EJ, Bowman BJ, Manolson NF, Poole RJ, DateT, OshimaT, Konishi J, Deda K, YoshidaM (1989) Evolution of the vacuolar H+-ATPase implications for the origin ofeukarotes. Proc Natl Acad Sci USA 86 6661-6665 Goksoyr J (1967) Evolution of eukaryotic cells. Nature 214 1167... 

Deda P. and Rubian R. (2004). Women and biodiversity The long journey from users to policymakers. Natural Resources Forum, 28 (3) 201-205. 

Neve F, Deda ML, Crispini A, Bellusci A, Puntoriero F, Campagna S (2004) Organometallics 23 5856... 

Support for the potential role of PWI in selecting patients for intravenous thrombolysis has come from several studies in which thrombolysis was found to be effective later than 4.5 h, if offered only to patients with a large-enough diffusion-perfusion mismatch. One such study required at least a 50% mismatch between lesions seen on DWI and TTP maps, and found that thrombolysis could be effective up to 6 h [74]. In the Desmoteplase In Acute Ischemic Stroke (DIAS) and Dose Escalation of Desmoteplase for Acute Ischemic Stroke (DEDAS) trials, thrombolysis was effective in patients presenting 3-9 h after onset, if offered only to those with at least a 20% mismatch between lesions seen on DWI, and on pseudo-MTT maps that were created using the normalized first... 

Polymer Deda Handbook. Copyright 1999 by Oxford University Press, Inc. All rights reserved. 

The density-based energy decomposition analysis (DEDA) method was recently developed by Wu et al. [38] for intermolecular interactions, as illustrated in Fig. 4.1. Similar to other energy decomposition analysis approaches, the determination of binding energy components between two isolated molecules - A (with density po, A) and B (with density po, B) - and their binding complex... 

Figure 4.1 Illustration of the density based energy decomposition analysis (DEDA) scheme. Reprinted with permission from Lu, Z., Zhou, N., Wu, Q. and Zhang, Y. Directional dependence of hydrogen bonds A density-based energy decomposition analysis and its implications on force field development. J Chem Theory Comput 7, 4038-4049 (2011). Copyright (2011) American Chemical Society.

The implementation of DEDA represents a critical difference from other EDA methods in that energies of all intermediate states are variationally determined, which to the best of our knowledge has not been achieved before. For the frozen density energy, the optimization is done through a constrained search formulation in DFT, i.e., [/o + = min E [p], and implemented with the Wu-... 

DEDA of the intermolecular interactions therefore involves four steps of variational calculations, all of which can be done with an in-house modified version of NWChem [43], As illustrated in Fig. 4.1, these four steps are (1) regular DFT calculations of the fragments (using all the basis functions in the complex), (2) a WY calculation for the frozen density of the complex that is built from the sum of fragments densities, (3) a constrained DFT calculation where charges are constrained to be the same as in the frozen density, and (4) a regular DFT calculation of the complex. 

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