DEAE-cellulose ion-exchange chromatography

DEAE-cellulose ion-exchange chromatography, which is dependent on the phosphate, pyrophosphate or phosphoethanolamine groups of the lipid A moiety, is widely used for lipid A isolation (El Hamidi et al., 2005 Raetz and Kennedy, 1973). TLC, as a lipids detection method, also can be applied for lipid A isolation (Zhou et al., 1999). In addition, the chromatography techniques based on the molecular... 

In 1965, Yonetani and Ray H) obtained a highly purified preparation of cytochrome c peroxidase in an excellent yield using DEAE-cellulose ion exchange chromatography. Shortly thereafter, Yonetani et al. 15) crystallized this enzyme by isoelectric dialysis. Subsequently, Yonetani and co-workers 16-40) carried out a series of extensive investigations... 

A proteinaceous inhibitor of rubber biosynthesis was purified from the C-serum of Hevea brasiliensis latex. The protein inhibited the incorporation of isopentenyl diphosphate into rubber. Purification was achieved by employing three column chromatography methods Sephadex G-150 gel filtration, DEAE-Cellulose ion exchange chromatography and Phenyl Sepharose CL-4B hydrophobic interaction chromatography. 21 refs. 

Mevalonic acid 5-pyrophosphate [1492-08-6] M 258,1, Purified by ion-exchange chromatography on Dowex-1 formate [Bloch et al. JBC 234 2595 7959], DEAE-cellulose [Skilletar and Kekwick, AB 20 171 7967], on by paper chromatography [Rogers et al. BJ99 381 7966]. Likely impurities are ATP and mevalonic acid phosphate. Stored as a dry powder or as a slightly alkaline (pH 7-9) soln at -20°. 

When the purified enzyme is subjected to ion-exchange chromatography (DEAE-cellulose) or gel filtration (Sephadex G-150), the three activities elute together with constant ratio in all fractions. 

Kalb683 subsequently separated the aforementioned protein preparation into three components by ion-exchange chromatography on DEAE-cellulose. Sedimentation equilibrium established the following molecular weights component A, 120,000 component B, 58,000 and component C, 117,000 (designated in order of salt elution from DEAE-cellulose at pH 7.6). 

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