Cutting sections

When this house was to be removed to provide a different scene (a main attraction for two decades), it had suffered almost no change in deflection. It was estimated to have been subjected to winds, earthquakes, subjected to families using it to the equivalent of centuries based on all the people that passed through it, etc. Destruction by conventional techniques (wrecking ball, etc.) was impossible without first cutting sections, etc. 

To obtain a clear picture of larger, more complex, offline WT boilers, in addition to visual inspections, it is common practice to trepan or cut sections out of some of the tubes. The deposits found are weighed, reported as a weight per unit surface area, and analyzed. Repairs are then made to the tubes. 

Once initiated, and provided the surface continues to be exposed to the environment, the process of hydration continues at a slow, but measurable rate. The adsorption of the water is accompanied by changes in the physical properties of the obsidian. The refractive index of the obsidian, for example, is altered as it becomes hydrated. If the obsidian was subjected to alternative wet and dry periods, successive hydrated layers are formed on the surface. The differences in refractive index between the bulk and the hydrated layer (or layers) creates an interface between the bulk and the hydrated layer, and between the layers, that stands out sharply when observing a cross-cut section of obsidian under a microscope (see Fig. 23). Thus the thickness of the hydrated layer, or layers, can be measured. 

Cutting sections at 3 pm facilitates single cell adherence to charged slides. The presence of nonadhered cells sloughing off between cell spots is a key characteristic of thick cell line sections (see Fig. 6.8). An awareness of actual... 

IV. Cutting Sections Ultramicrotomes are designed to cut ultrathin sections, semithin sections and ultrathin frozen sections if suitably... 

Cut sections and stain with uranyl acetate and lead citrate. 

Figure 4. Sections of the Plastofrost sample showing the ceramic tubes holding the thermocouples Left cut section, but not impregnated Right polyester impregnated section.

Before starting cutting sections from the tissue blocks, the whole workstation (bench top, microtome, blade holder, brushes, tweezers, cooling plate, water bath, etc.) should be cleaned with RNase ZAP. Set the cooling plate to -15°C and place the FFPE block on the plate. 

Cut sections 5-pm thick and mount on coated glass slides. Dewax the slides in xylene for 3 x 20 min and then rehydrate in 100% ethanol (2x2 min), then 95% and 70% ethanol and water for 2 min each... 

Cut sections 5-pm thick and mount on coated microscope slides. The drying temperature must not exceed 37°C, as higher temperatures cause denaturation of the antigen (see Note 5). 

Figure E3.1.2 (A) Hand-cut section from fresh apple tissue stained with phloroglucinol/HCl. Cell walls of the xylem tracheary elements (spirals) are stained red indicating they are lignified. (B) Increased magnification of A. (C) Hand-cut section from fresh pear tissue stained with phloroglucinol/HCl solution. Walls of the sclereid cells (stone cells center) are stained red indicating they are lignified. This black and white facsimile of the figure is intended only as a placeholder for full-color version of figure go to http //www.currentprotocols.com/colorfigures...

To cut sections of the thickness indicated and to observe than more easily, suitable forms of apparatus have been constructed,... 

In addition to high-speed photography, which was used to obtain the results discussed above, other methods have been used to visualize flow, for example, the tracer method.279"281 A typical experimental device consists of a capillary viscometer connected to a mold, which is a simple model of plunger-type injection molding unit.279 A special device was used to introduce tracers of different colors in regular succession. After sample solidification, it is possible to examine the position of the various colors on the surface (Fig. 4.47 a) or through the volume by cutting sections (Fig. 4.47 b). It can be seen that the tracers are positioned symmetrically near the surfaces and that they have a V-type shape, with the tip oriented toward the stream. 

Fig. 2.30 Topological view of subcutaneous rat tissue coated with particulate barrier dressing after 60 s application (cut section for inspection)...

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