Cuscuta Extract

Gupta M, Mazumder UK, Pal DK, Bhattacharya S Anti-steroidogenic activity of methanolic extract of Cuscuta reflexa roxb. stem and Corchorus olitorius Linn, seed in mouse ovary. Indian J Exp Biol 2003 41 641-644. 

Cuscutic resinoside A (1 tetradecanoic acid, (115)-[[6-deoxy-3-(9-(6-deoxy-a-L-mannopyranosyl)-4-0-[(2/ ,3R)-3-hydroxy-2-niethyl-l-oxobutyl]-a-L-nianno-pyranosyl]oxy]-intramol. l,2 -ester) was obtained from the ethyl acetate-soluble fraction of a methanol extract prepared from the seeds of Cuscuta chinensis Lam. The purification of this compound employed a combination of column and preparative-scale HPLC. The structure was deduced from spectroscopic evidence and acid hydrolysis 14). The degradative process gave convolvuUnolic acid, nilic acid, and L-rhamnose. The sugar components were identified by GC analysis after being converted to their thiazolidine derivatives. This disaccharide has a unique macrocyclic lactone, which is placed between C-1 and C-2 of the first rhamnose moiety. 

N1 - CUSCUTA REFLEXA Roxb., extract excluding root ... 

Plant tissue collection for DNA and protein studies has primarily utilized leaf tissue, but seeds, roots, flowers, stems, pollen, spores, and game-tophytes have all been used successfully. For example, DNA extraction from the parasitic Cuscuta required using only intemode tissue to prevent DNA contamination from its host species26 likewise, the green stems of a leafless Koeberlinia provided an adequate source of DNA.27... 

Dried aerial parts of C. microphylla (CH3OH extract) yielded 5 x 10 % dried powdered roots of C. nepalensis were extracted with water, chromatographed on polyamide and on silica gel yielding 2 x 10 % dried leaves of L. parasiticus extracted with CjHsOH yielded 2 x 10 % dried seeds of Cuscuta japonica were extracted with C2H5OH, partitioned and chromatographed yielding 1 x 10 " ... 

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