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Crystallization, X-ray analysis, and the tertiary structure

The crystallization of native elongation factor Tu isolated from bacterial cells, i.e. in complex with GDP, proved to be difficult. Useful crystals for X-ray structure analyses could be obtained only from a partially trypsinized E. coli EF-Tu GDP missing a part of the L2 region (amino acid residues 45-58 E. coli numbering). Through laborious efforts of several crystallographic groups, the structure of the partially proteolyzed E. coli EF-Tu GDP has been determined up to 2.5 A resolution [56]. [Pg.386]

The structure of EF-Tu in the active GTP form, namely that of EF-Tu GppNHp from T thermophilus, was determined up to 1.7 A resolution [1]. The EF-Tu structures in GDP and GTP bound forms can now be compared (Fig. 19.12). However, we still have to keep in [Pg.386]


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Analysis crystal

Crystal structure analysis

Crystal x-ray

Crystallization analysis

Crystallization and structure

Crystals and crystal structures

Ray Structural Analysis

Structures Tertiary structure

Tertiary structure

The Crystal Structure

X crystal structure

X-Ray crystal analysis

X-ray crystal structure

X-ray crystal structure analysis

X-ray crystallization

X-ray structure analysis

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