Cryo-sectioning

Walther P and Muller M 1999 Biological structure as revealed by high resolution cryo-SEM of block faces after cryo-sectioning J. Microsc. 196 279-87... 

Laman ID, Kors N, Heeney JL, et al. Fixation of cryo-sections under HIV-1 inactivating conditions integrity of antigen binding sites and cell surface antigens. Histochemistry 1991 96 177-183. 

Slot, J.W., and Geuze, H.J. (1984) Cold markers for single and double immunolabeling of ultrathin cryo-sections. In Immunolabeling for Electron Microscopy (J.M. Polak, and I.M. Varndess, eds.), p. 139. Elsevier, New York. 

Sjostrom, M., and Squire, J. M. (1977). Fine structure of the A-band in cryo-sections. The structure of the A-band of human skeletal muscle fibres from ultra-thin cryo-sections negatively stained./. Mol. Biol. 109, 49-68. 

Conventional bright-field TEM observations of polyolefins often require contrast enhancement, usually by staining with Ru04 or other suitable markers [17]. These accumulate in the amorphous phase, at lamellar surfaces and in cavities, and differential staining can reveal the phase distribution in blends. Staining also hardens the specimens, facilitating preparation of thin sections at room temperature (cryo-sectioning is required for unfixed polyolefins). 

Ullberg, S. (1977). The technique ofwhole-body autoradiography. Cryo-sectioning of large specimens. Science Took (The LKB Instr. Journd). 2-29 (special issue)... 

The animals were sacrificed after the lead free period. The eyes were immediately enucleated and hemisected along the ora serrata. The vitreous was removed and the eye cup was fixed in 2% paraformaldehyde, 2% glutaraldehyde in O.IM phosphate buffer (PB, pH 7.4) for 24 hours. The fixed retina was dissected, washed in PB and prepared for cryo sectioning. 

Figure 6.1 Dependence of water vapour absorption features in single channel mid-IR spectra with (left panel) and without (right panel) purging of the microscope sample compartment by dry air. Spectra were taken in transmission mode from real-world samples (cryo-sections of colon tissue mounted on Cap2 windows). Note the sharp and intense water vapour absorption bands in the spectral regions 1350-1950 and 3600-3900cm , with nearly total absorption between 1500 and 1800cm (no purge box,...

Figure 6.5 Spectral features as a function of the apodisation function in mid-IR microspectroscopy of tissues (colon tissue cryo-section, same sample position as in Figure 6.4). Transmission type spectra were acquired using Bruker s IRScope II microscope and an IFS28/B spectrometer. Further measurement parameters aperture diameter 900 pm, Cassegrain objective (36 x, NA 0.5), 128 scans, optical substrate CaF2 pm of 1 mm thickness. Spectral resolution 6 cm zero-filling factor (ZFF) 4. Transmission spectra were processed with a first derivative Savitzky-Golay filter with nine smoothing points.

Figure 11.8 (A) Photomicrographs of unstained cryo-sections (first and third columns) and corresponding FTIRM images (second and fourth...

Cryo-fixation followed by cryo-sectioning to a thickness of 5-10 pm and freeze-drying on thin (<1 pm) plastic films Small particles on filters analysis in situ on filter membrane. 

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