CPG, controlled pore glass

CPG = Controlled Pore Glass (solid support) 1 and 2 (Bj, B2, B3, B4), commercially available... 

Figure 4.16 — Schematic diagram of a split-stream FI system used for the determination of glutamine in bioreactor media C de-ionized water carrier stream R buffer diluent reagent stream S sample injection point L delay coils CPG controlled pore glass enzyme reactor ISE ammonium ion-selective membrane electrode W waste. (Reproduced from [139] with permission of the American Chemical Society).

A dry packed column with porous material was used for the characterization according to size of the PVAc latex samples. The packing employed was CPG (Controlled Pore Glass), 2000 A, 200-400 mesh size. Deionized water with 0.8 gr/lit Aerosol O.T. (dioctyl sodium sulphosuccinate), 0.8 gr/lit sodium nitrate and 0.4 gr/lit sodium azide served as the carrier fluid under a constant flowrate. The sample loop volume was 10 pC A Beckman UV detector operating at 254 nm was connected at the column outlet to monitor particle size. A particle size-mean retention volume calibration curve was constructed from commercially available polystyrene standards. For reasons of comparison, the samples previously characterized by turbidity spectra were also characterized by SEC. A number of injections were repeated to check for the reproducibility of the method. 

Diagnosis of renal problems, xanthinuria, and toxemia of pregnancy via determination of the ratio of hypoxanthine to xanthine in plasma is facilitated by the use of biosensors. Xanthine oxidase immobilized on aminopropyl-CPG (controlled pore glass) activated with glutaraldehyde oxidizes hypoxanthine first to xanthine and then to uric acid. Use of an IMER with biosensors for hypoxanthine, xanthine, and uric acid provides the necessary data. Pre- or postcolumn enzymatic reactions catalyzed by creatinine deiminase, urease, alkaline phosphatase, ATPase, inorganic pyrophosphatase, or arylsufatase facilitate analysis of uremic toxins (simultaneous detection of electrolytes, serum urea, uric acid, creatinine, and methylguanidine). 

The silica-based supports are supplied by Bio-Rad Labs (Richmond, CA, U.S.A., under the trademark Bio-Glass) by Electro-Nucleonics, Inc. (Fairfield, NJ, U.S.A.) and Pierce Chem. Co. (Rockford, IL, U.S.A. under the name CPG (controlled-Pore Glass)) by Waters Associates (Milford, MA, U.S.A. under the name Porasil) by E. Merck (Darmstadt, G.F.R., under the names Fractosil or LiChrospher) by DuPont Instrument Products Div. (Wilmington, DE, U.S.A., under the name SE Series or Zorbax), or by Rhone Poulenc (France, under the name Spherosil). The modified silica-based supports are supplied by Electro-Nucleonics, Inc. (Fairfield, NJ, U.S.A.) or Pierce Chem. Comp. (Rockford, IL, U.S.A., under the trademark Glycophase-... 

DMTr 4,4 -dimethoxytiityl B thymine or any N-piotected nucleobase CPG controlled pore glass... 

CPG controlled pore glass DMT 4, 4 -dimethoxyltritylchloride TBDMS t-butyldimethylsilyl group... 

Abbreviations. Donors A= Trichloroacetimidate B=ThioelkyI/aryI C= Sulfoxide D= n>Pentenyl E=Fluoride F= Glycal Polymers CPG= Controlled-Pore Glass TG TentaGel PS(M)= Merrifleld Resin ... 

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