Corynebacterium glutamicum, industrial

The fermentation wastes Corynebacterium glutamicum biomass) were obtained in a dried powder form from a lysine fermentation industry (BASF-Korea, Kunsan, Korea). The protonated biomass was prepared by treating the raw biomass with a 1 N HNO3 solution for 24 h, thereby replacing the natural mix of ionic species with protons. The resulting C glutamicum biomass was dried and stored in a desiccator and used as a biosorbent for the sorption experiments. 

Glutamate Corynebacterium glutamicum, Brevibacterium spp. Food industry as flavour-accentuating agent... 

Hayashi M, Ohnishi J, Mitsuhashi S et al (2006) Transcriptome analysis reveals global expression changes in an industrial L-lysine producer of Corynebacterium glutamicum. Biosci Biotechnol Biochem 70 546—550... 

The production of L-malic acid (2) from fumaric acid (1) is carried out by the enzyme fumarate hydratase (E.C. 4.2.1.2), which is part of the tricarboxylic acid cycle and ubiquitous in nature (Scheme 11.5-1). The product is used in food, pharmaceutical and cosmetic industries and is produced at a multi-ten-tonne scale. Although the enzyme can be applied in isolated form, as performed by Tanabe, the use of whole cells of Corynebacterium glutamicum has been reported by Amino GmbH as well141. 

Part B Multipurpose Bacterial Cell Factories offers four chapters on some of the most widely used industrial microorganisms. Escherichia coli (Chapter 5), Corynebacterium glutamicum (Chapter 6), Bacillus subtilis (Chapter 7), Pseudomonas putida (Chapter 8) have evolved into synthetic platforms with a broad range of applications. Their product portfolios include fine chemicals, bulk chemicals, drugs, flavors and fragrances, materials, fuels, therapeutic and diagnostic... 

Amino acids, citric acid, lactic acid, propanediol, penicillin G, synthetic drug intermediates, and therapeutic proteins are among the industrially relevant products of fermentation and cell culture that have been targets for metabolic engineering. Some ofthis work has been adopted by industry (see [72], section 16.4.1). The major aim was to optimize the yields of industrial products, which was efficiently realized with Corynebacterium glutamicum for lysine and tryptophan, and at the Dupont company for 1,3-propane diol production [107-109]. 

Figure 6.2 Timeline of research and development of the industrial, Gram-positive soil bacterium Corynebacterium glutamicum, highlighting major discovery breakthroughs in the microbiology, genetic, omics, and...

Table 6.4 Overview on the industrial amino acid market [96] and the production performance of Corynebacterium glutamicum [223, 230, 237].

C., Sivalingam, J., Grunberger, A., Kalinowski, J., Bott, M., Noack, S., and Frunzke, J. (2013) Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 for use as a platform strain for basic research and industrial biotechnology. Appl. Environ. Microbiol, 79 (19), 6006—6015. 

---