Content in tissues

Horiguchi, T., Nishikawa, T., and Ohta, Y. et al. (2007). Retinoid X receptor gene expression and protein content in tissues of the rock sheU Thais clavigera. Aquatic Toxicology 84, 379-388. 

Thatheyus, A.J., M. Selvanayagam, and S.S. Raja. 1992. Toxicity of nickel on protein content in tissues of Cyprinus carpio communis (Linn). Indian Jour. Environ. Health 34 236-238. 

Co deficit Everywhere Low content of Co in Podsoluvisols, Podzols, Arenosols and Histosols. The average Co content in plant species is < 5 ppb The decrease of Co content in tissues decrease of vitamin BJ2 in liver (tr.—130 ppm), in tissue (tr.—0.05 ppm), in milk (tr.—3 ppm). Synthesis of vitamin Bi2 and protein is weakened. Cobalt-deficiency and Bj2 vitamin-deficiency. The number of animal diseases is decreasing in raw sheep —cattle — pigs and horses. Low meat and wool productivity and reproduction... 

Williams UP, Kiceniuk JW, Fancey LL, et al. 1989. Tainting and depuration of taint by lobsters Homarus americanus) exposed to water contaminated with a no. 2 fuel oil Relationship with aromatic hydrocarbon content in tissue. J Food Sci 54(2) 240-243. 

Chromium Content in Tissues and Body Fluids of the General Population... 

On supplementing SeMet, GSH-Px activities reach a maximum value and then plateau, while tissue Se levels continue to rise in proportion to the dosage (Deagen et al, 1987). The release of Se from SeMet also depends on vitamin B6 status. In rats maintained on a feed supplemented with 0.25 mg Se/kg of diet in the form of SeMet, GSH-Px activities in erythrocytes, muscle and heart were lower in vitamin B6-deficient than in vitamin B6-supplemented animals (Yin et al, 1996). Vitamin B6 also significantly reduced lipid peroxide contents in tissues. These results indicate that dietary vitamin B6 is involved in the metabolic breakdown of SeMet. 

The peptide concentration in the individual specimens is determined by radioimmunoassay, using a synthetic reference peptide for the standard curve. Several commercial assays are now available for hypothalamic peptides. An alternative method is the determination of peptide content in tissue extracts by an HPLC method or by mass spectrometry. 

Determination of Ascorbic Acid. A 500-ftL aliquot of plasma was mixed with 4.5 mL of 5% metaphosphoric acid followed by analysis in an autoanalyzer using a modified fluorometric method (13). Ascorbic acid content in tissues was determined according to a published method (14). 

The semiquantitative and quantitative methods discussed in this section are based on the optical properties of melanins in both the transmission and reflection mode, and they may require solubilization and/or partial degradation of the samples (305). The melanin content in tissues has been determined visually (306) following treatment with Fe and potassium ferricyanide (307) and by reflectance (308) and remittance (143) methods. For fluorimetric determination of melanins (melanoma cells), the sample is solubilized with alkaline hydrogen peroxide (pH 7.8, 100°C, 30 min) the excitation wavelength is 410 nm, emission 500 nm (148). Fairly good chemical stability of melanins has been determined in gravimetric determinations after separation from all other constituents of melanosomes with acid digestion (6 N HCl, 100°C, 72 hr) (110). 

DEVELOPMENT OF REFERENCE STANDARDS AND STANDARD CURVES FOR CALCULATION OF ANTIGEN CONTENT IN TISSUE SECTION... 

The packaging processes that accompany apoptosis, and which are currently used to identify this phenomenon, may therefore be a late event to either aid digestion of the dying cell after it has been phagocytosed or to prevent spillage of cellular contents in tissues where phagocytosis may not... 

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