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Confocal microscope real time

Fig. 9 Surface modification of cells with ssDNA-PEG-lipid. (a) Real-time monitoring of PEG-lipid incorporation into a supported lipid membrane by SPR. (r) A suspension of small unilamellar vesicles (SUV) of egg yolk lecithin (70 pg/mL) was applied to a CH3-SAM surface. A PEG-lipid solution (100 pg/mL) was then applied, (ii) Three types of PEG-lipids were compared PEG-DMPE (C14), PEG-DPPE (C16), and PEG-DSPE (C18) with acyl chains of 14, 16, and 18 carbons, respectively, (b) Confocal laser scanning microscopic image of an CCRF-CEM cell displays immobilized FITC-oligo(dA)2o hybridized to membrane-incorporated oligo(dT)20-PEG-lipid. (c) SPR sensorigrams of interaction between oligo(dA)2o-urokinase and the oligo (dT)2o-PEG-lipid incorporated into the cell surface, (i) BSA solution was applied to block nonspecific sites on the oligo(dT)20-incorporated substrate, (ii) Oligo(dA)20-urokinase (solid line) or oligo(dT)20-urokinase (dotted line) was applied... Fig. 9 Surface modification of cells with ssDNA-PEG-lipid. (a) Real-time monitoring of PEG-lipid incorporation into a supported lipid membrane by SPR. (r) A suspension of small unilamellar vesicles (SUV) of egg yolk lecithin (70 pg/mL) was applied to a CH3-SAM surface. A PEG-lipid solution (100 pg/mL) was then applied, (ii) Three types of PEG-lipids were compared PEG-DMPE (C14), PEG-DPPE (C16), and PEG-DSPE (C18) with acyl chains of 14, 16, and 18 carbons, respectively, (b) Confocal laser scanning microscopic image of an CCRF-CEM cell displays immobilized FITC-oligo(dA)2o hybridized to membrane-incorporated oligo(dT)20-PEG-lipid. (c) SPR sensorigrams of interaction between oligo(dA)2o-urokinase and the oligo (dT)2o-PEG-lipid incorporated into the cell surface, (i) BSA solution was applied to block nonspecific sites on the oligo(dT)20-incorporated substrate, (ii) Oligo(dA)20-urokinase (solid line) or oligo(dT)20-urokinase (dotted line) was applied...
Fig. 2. Diagram of the optical path of the bilateral laser scanning confocal microscope (InSIGHT). Real-time confocal imaging is obtained through the use of a double-sided mirror which simultaneously scans the sample and the oculars or detector at video rates. BSO, beam shaping optics LM, laser mirror D, dichroic M4, mirrors SM, scanning mirror G, galvonometer L1-L3 lenses S, variable slit. Fig. 2. Diagram of the optical path of the bilateral laser scanning confocal microscope (InSIGHT). Real-time confocal imaging is obtained through the use of a double-sided mirror which simultaneously scans the sample and the oculars or detector at video rates. BSO, beam shaping optics LM, laser mirror D, dichroic M4, mirrors SM, scanning mirror G, galvonometer L1-L3 lenses S, variable slit.
Variations of the confocal design have been developed, such as bilateral scanning in real time with a scanning slit [71]. Because of the fast scanning, TV detectors such as a sensitive CCD camera are best suited. Slit-scan confocal microscopes offer a better signal-to-noise ratio and real-time imaging capabilities but a slightly reduced resolution compared to a conventional CLSM. [Pg.1073]

This chapter starts by describing the ubiquitous problems in optics, in particular, those that can be solved by liquid microlenses. Then we will discuss the benefits and advantages of liquid microlenses in many applications artificial implementation of compound eyes endoscopic fiber microscopes for confocal reflectance and real-time optical coherence tomography (OCT) photolithography optical communications imaging systems and labs on chips. [Pg.1]

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