Concanavalin complex formation

The molecular recognition of septanose carbohydrates has been investigated in depth by using concanavalin A68 as a model lectin. Complex formation was analysed by STD experiments and showed the first direct evidence of binding, by a natural protein, for this class of ring-expanded carbohydrate molecules. 

Complexes, see also specific type in solution, structures, see X-ray diffraction n-Complexes, 4 178-184 Complex formation constant, outersphere, 43 46, 55 electrovalent interaction in, 3 269-270 Compressibility coefficient of activation, 42 9 Comproportionation constants, class II mixed-valence complexes, 41 290-292 Comproportionation equilibrium, 41 280-281 Compton effect, 3 172 Conantokins, calcium binding, 46 470-471 Concanavalin A, 36 61, 46 308 Concensus motif, 47 451 Concentration-proportional titrations of poly-metalates, 19 250, 251, 254 Condensation... 

Insoluble concanavalin A has already been used in the purification of glycoproteins,and, in view of its commercial availability, it will no doubt prove useful in structural, studies. Furthermore, the in-solubilized lectin may be used in column form for the separation of monosaccharide mixtures and of polysaccharide mixtures " it has been demonstrated that mixtures of carbohydrates not separable by complex-formation with concanavalin A in solution may be separated by using the immobilized form,... 

Carbohydrate—protein complex-formation. Some factors affecting the interaction of D-glucose and polysaccharides with concanavalin A. Carbohydr. Res. , 89-100. 

Nakamae K, Miyata T, Jikihara A, and Hoffman AS. Formation of poly(glycosyloxyethyl methacrylate)-concanavalin A complex and its glucose sensitivity. Journal of Biomaterials Science, Polymer Edition 1994 6 79-90. 

Nucieotidases.—Since the 5 -nucleotidase from Dictyostelium discoideum interacted with immobilized concanavalin A, it appears to be a glycoprotein. Pectinesterases.—The occurrence, formation, assay, purification, and specificities of pectinesterases have been reviewed. Pectinesterase activity was detected both in healthy and diseased onions Allium cepa), but not in cultures of the invading bacterium (Pseudomonas cepacia) Both the pectinesterase and poly-D-galact-uronate lyase activities of Clostridium multifermentans are associated with a single complex. ... 

A., and Hoffman, A.S. (1994) Formation of poly(glucosyloxyethyl methacrylate)-Concanavalin A complex and its glucose-sensitivity. J. Biomater. Sci., Polym. Ed., 6, 79-90. 

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