Composite cryogels

Gelatine + acrylonitrile Glutaraldehyde Glutaraldehyde, Water -12 -12 for the immobilization yeast cells Preparation of macroporous scaffolds for tissue engineering Preparation of composite cryogel via [185] [186]... 

Podorozhko EA, D yakonovaEA, Kolosova OY, KlabukovLF, Lozinsky VI (2012) A study of cryostructuring of polymer systems. 34. Poly(vinyl alcohol) composite cryogels filled with microparticles of polymer dispersion. Colloid J 74 708... 

Podorozhko EA, Korlyukov AA, Lozinsky VI (2010) Ciyostmctuiing of polymer systeans. XXX. Poly(vinyl alcohol)-based composite cryogels filled with small disperse oil droplets a gel system capable of mechanically induced releasing of the lipophilic constituents. J Appl Polym Sci 117 1332. 

Keywords Cell chromatography Immobilized cells Molecular imprinting Cell bioreactors Composite cryogels... 

The polymer backbone is often rather inert. In order to make it useful for separation purposes one needs to introduce functionalities. This can be done by adding specific monomers to the mixture that is to be polymerized, or one can introduce functimi-ality afterwards. A third variant is to add particles with functionality and they become incorporated into a composite cryogel. 

Entrapment of adsorbent particles within a cryogel has been studied as a method to improve the binding capacity. There are also some cases where entrapment was carried out because the particles were too small to handle in conventional separation and the composite strategy was preferred. Table 2 lists some examples where composite cryogels have been used for bioseparation. Besides an increase in... 

From Table 2, it can be seen that a range of different materials have been used to create composite cryogels. An extreme case is the entrapment of microemulsions, which led to a preparation with one solid and one liquid phase with very different properties [79]. 

Xu P, Yao Y, Shen S, Yun J, Yao K (2010) Preparation of supennacroporous composite cryogel embedded with Si02 nanoparticles. Biotechnol Bioeng Chin J Chem Eng 18 667-671... 

Hajizadeh S, Kirsebom H, Leistner A, Mattiasson B (2012) Composite cryogel with immobilized concanavalin A for affinity chromatography of glycoproteins. J Sept Sci 25 2978-2985... 

Andac M, Baydemir G, Yavuz H, Denizli A (2012) Molecularly imprinted composite cryogel for albumin depletion from human serum. J Mol Recognit 25 555-563... 

Another material that has been added to PVA to produce a composite cryogel is chitosan. Chitosan is obtained from chitin by alkaline deacetylation. It is a cationic polysaccharide, and has been proposed as a good material for addition to the PVA matrix for cryogel composite production in order to enhance protein absorption [79]. Due to the hydrophilicity of PVA, cell adhesion proteins are not able to absorb, preventing cell adhesion [79]. Because of this, work to create a more favorable environment for cell growth while still maintaining the beneficial mechanical properties of the PVA cryogel structure is important for certain applications. 

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