Complexity biotechnology applications

We continue our attempts to gain some control over the construction of D. vulgaris bidirectional hydrogenase in E. coli not only to come to an understanding of the biosynthesis of complex Fe/S enzymes, but also to bring the candidacy of this protein for biotechnological applications within the realm of possibility. 

The enzyme vanillyl-alcohol oxidase (VAO, E.C. 1.1.3.38) was examined in detail with respect to mechanism, structural properties, and biotechnological applications by van Berkel and coworkers, giving an excellent example of how detailed biochemical studies provide a basis for preparative biocatalytic applications (for recent reviews see[1, 21). The homooctamer with a monomer mass of 65 kDa was isolated and purified from Penicillium simplicissimum. The catalytic mechanism of VAO-catalyzed oxidation of para-alkyl phenols was studied in detail[3-5 After initial hydride abstraction from the Ca atom, a binary complex of the intermediate para-quinone methide and reduced FAD reacts with molecular oxygen, regenerating the... 

The possibilities of using the photosynthetic systems immobilized in many biotechnological applications can explain the great interest carried to the various methods of stabilization. The photosystems are enzymatic complexes which are subjected to the effects of various pollutants. Among others, the toxic substances found in icultural, industrial, and municipal effluents can deteriorate PSII in the photosynthetic organisms of aquatic and terrestrial biomass. The use of photosynthetic materials as biological receptors in a biosensor is an excellent tool for the detection of many pollutants. 

The decision of when to file a patent application can be tricky for complex biotechnological inventions that involve a long research and development period. In the case of the NF-kB patent application, it took 16 years to complete the review at the USPTO before the patent was issued [87]. This significant review period was, at least in part, caused by the large number of claims that were required because of the complexity of the invention. 

Zhang, Y.H. (2011) Substrate channeling and enzyme complexes for biotechnological applications. Biotechnol Adv., 29, 715-725. 

Luengo JM, Garcia JL, Olivera ER (2001) The phenylacetyl-CoA catabolon a complex catabolic unit with broad biotechnological applications. Mol Microbiol 39 1434-1442 Luengo JM, Garda B, Sandoval A, Naharro G, Olivera ER (2003) Bioplastics from microoiganisms. Curr Opin Biotechnol 6 251-260... 

Recombinamers Combining Molecular Complexity with Diverse Bioactivities for Advanced Biomedical and Biotechnological Applications... 

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