Competitive fluoroimmunoassay

In aqueous buffer, 500 mg of polymer were required to bind 50% of the CMMC, whereas only 100 mg were needed to sequester 50% of the C-2,4D. In 

Toward optical sensors for biologically active molecules 

Competitive binding experiments in the presence of 0.64 nM CMMC supported these findings. Based on the fluorescence of the supernatant, 2,4-D could be detected from 0.1 to 50 /tM in phosphate buffer and from 0.1 to 10 yuM in MeCN. The previous MIP-based radioimmunoassay, carried out in the same buffer, was able to detect the analyte over a similar range, from 0.135 to 45 /iM. As with the work of Piletsky et al. (see Sections 20.2.5.2. and 20.2.5.3.), the lengthy incubation time is a disadvantage of such systems. 

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Haupt K, Mayes AG, Mosbach K. Herbicide assay using an imprinted pol3mier-based system analogous to competitive fluoroimmunoassays. Anal Chem 1998 70 3936-3939. 

Figure 7.10. Standard curves for 2,4-dichlorophenoxyacetic acid (2,4-D).Displacement of CMMC from the 2,4-D-imprinted MIP by 2,4-D (I), CPOAc ( ) and POAc (A) in a) 20 mM phosphate buffer, pFf 7, 0.1% Triton X-100 b) acetonitrile. Reprinted with permission from Haupt K, Mayes AG, Mosbach K. Herbicide assay using an imprinted polymer-based system analogous to competitive fluoroimmunoassays. Anal Chem 1998 70 3936-3939. 1998 American Chemical Society...

Cummins CM, Koivunen ME, Stephanian A, Gee SJ, Hammock BD, Kennedy IM (2006) Application of europium(III) chelate-dyed nanoparticle labels in a competitive atrazine fluoroimmunoassay on an ITO waveguide. Biosens Bioelectron 21 1077-1085... 

B. J. Tromberg, M. J. Sepaniak, T. VoDinh, and G. D. Griffin, Fiber-optic chemical sensors for competitive binding fluoroimmunoassay, Anal. Chem. 59, 1226-1230(1987). 

Dechaud H, Bador R, Claustrat F, Desuzinges C, MaUein R. New approach to competitive lanthanide immunoassay Time-resolved fluoroimmunoassay of progesterone with labeled analyte. Clin Chem 1988 34 501-4. 

Among homogeneous fluoroimmunoassays based on conventional fluorimetry, substrate-labeled fluoro-immunoassay is another alternative for both hapten and protein determination using a fluorescent label. As in other homogeneous competitive immunoassays, the sensitivity is limited by the serum background fluorescence and the limited amount of tracer that can be used. 

Griffin Fiberoptic Probe for Competitive Binding Fluoroimmunoassay," Anal. Chem. 59 (1987)... 

Tromberg B.J., Sepaniak M.J., Vo Dinh T. and Griffin G.D. (1987) Fiber-optic chemical sensors for competitive finding fluoroimmunoassay. Anal Chem., 59, 1226-1230. 

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