Competitive binding assay principle

Zettner, A. Principles of Competitive Binding Assays (Saturation Analyses) I Equilibrium Techniques. Clin. 

Alternatively, MIPs have also been used in biological receptors for competitive binding assays. The assay principle is similar to that in other known biological assays such as radioimmunoassay (RIA) or enzyme-linked immunosorbent assay (ELISA) except that instead of antibodies, MIPs are utilized. This method is often called molecularly imprinted assay (MIA). Typically, in MIA methods, a marker molecule (a labeled analyte analogue) is incubated together with the sample and the MIPs. Analyte and marker molecules compete for the binding... 

CLIA is similar to EIA and ELISA techniques except that the final receptor enzyme assay is replaced with a chemiluminescent tracer followed by measurement of light released as a result of the chemical reaction. The principles of a chemiluminescence competitive binding assay are shown in Figure 8-8. 

A Zettner, PE Duly. Principles of competitive binding assays (saturation analysis) II. Sequential saturation. Chn Chem 20 5, 1974. 

Figure 10.6 (A) Principle of CE-based competitive binding assay using on-capillary...

In CE, analysis by immunoassays depends on the principles that the antigen and antibody migrate differently when they are bound compared to when they are free. One of these two compounds (mostly the antigen) is labeled with a fluorescent tag. The unknown sample is mixed with labeled antigen for competitive binding assay and the mixture is separated by CE. The label in the bound fraction (or... 

Odiell, W. D. and Daughaday, W. Principles of Competitive Protein-Binding Assays. J. B. Lippincott (1971). 

Plates might be coated at the same time with MAbs of different specificity—individually or in combination with others. The principle of MAbs combination depends on whether there is some preliminary information about their mutual relationship, for example, competitive assay or peptide binding assay. Afterwards, the plates would be sequentially incubated with the same library preparation. 

Morris DL, Ledden DJ, Boguslaski RC (1987) Dry-phase technology for immunoassays. J Clin Lab Anal 1 243-249 Moss AJ, Dalrymple GV, Boyd CM (1976) Practical Radioimmunoassay. The C.V. Mosby Company, St. Louis Odell WD, Franchimont P (1983) Principles of Competitive Protein-Binding Assays. John Wiley and Sons, New York Read B (2001) Long-distance runners of the fluorophore family. Amer Biotechnol Lab 44... 

The binding assays that are performed in this flow injection system may be either competitive or sandwich type. An assay for the serum protein transferrin using a competitive ELISA (129) illustrates the principle (Fig. 5). An immunoafiinity purified polyclonal antiserum raised in rabbits against human transferrin is the binder. The immobilized antibody is packed in a small column (100-200 pL), which is placed in a continuous flow of buffer. The experimental setup is shown in Fig. 6. 

Odell, W.D. Daughaday, W.H. Principles of Competitive Protein Binding Assays Lippincott Philadelphia 1971. Christian, G.D. Clinical chemistry. Analytical Chemistry John Wiley and Sons Inc. New York, 1994 611-628. Ullman, E.E., Langen, J., Clapp, J.J., Eds. Liquid Assay Analysis of International Development on Isotopic and Non-Isotopic Immunoassay Masson New York, 1981 113. Sharma, A. Schulman, S.G. Fluorescence analytical methods and their applications. Introduction to Fluorescence Spectroscopy, WHey-lntexsdeace.l ew York, 1999 123-158. 

Campfield, L.A. Mathematical analysis of competitive protein binding assays. In Principles of Competitive Protein Binding Assays, 2nd Ed. Odell, W., Franchimont, P., Eds. John Wiley Sons New York, 1983 125-148. 

GE Abraham, PK Grover. Covalent linkage of hormonal haptens to protein carriers for use in radioimmunoassay. In WD Odell, WH Daughaday, eds. Principle of Competitive Protein-Binding Assays. Philadelphia Lippincott, 1974, p 134. 

The methods commonly known as binding assays are shown in the last two rows, divided into noncompetitive and competitive assays. The principles behind... 

Immunoassays, specific protein-binding assays, and radioisotope tests are sometimes used for the determination of water-soluble vitamins. These are the only feasible and practical methods for the quantification of certain vitamins in physiological samples. The principle of competitive protein binding (CPB) using labeled radioactive or fluorescent tracer is still routinely applied to quantification of serum vitamin... 

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