Competitive assay metabolites from

We developed a magnetic beads-based detection system for competitive assay of metabolites from OPs (Figure 3) (4J). This method employed a sequentially injecting washing buffer, a mixture of TCP (chlorpyrifos metabolite) containing sample,... 

An antibiotic inhibition zone often appears around Trichoderma spp. interacting with other fungi. The genus contains many species which produce secondary metabolites. Claydon et al. (23) have identified an antibiotic from T. harzianum as a volatile, 6-n-pentyl-2H-pyran-2-one this was recently shown to be an active antibiotic from T. koningii (24). The volatile appeared to be the factor responsible for the coconut smell of some biocontrol-effective strains of T. harzianum (25). However, in a Petri-plate assay, it can be difficult to be certain that antibiosis is involved. As well as competitive growth, lytic enzymes could also contribute to the action and Trichoderma has been shown to produce / -l,3-glucanase and chitinase (26-29). 

The application area of LC-MS is rapidly growing. LC-MS is now regularly used for the analysis of many different types of compound drugs and metabolites, herbicides-pesticides and metabolites, surfactants, dyes, saccharides, lipids-phospholipids, steroids, and many others. In our opinion, the area that profits more from the development of LC-MS is bioanalysis natural products, proteins, peptides, nucleosides, and metabolic studies. Despite the current trends toward immunoassays-biospecific assays and capillary electrophoresis, LC-MS is an extremely powerful analytical technique that is considered complementary to the above mentioned, rather than competitive. 

---