Comparison with conventional immunization

The conclusions that can be drawn from the experimental evidence reported above can be summarized as follows. First, laboratory mice maintained in a conventional animal house display, in comparison with mice kept in a protected low stress environment, display distinct humoral and immunity characteristics of exposure to stress that accompany significant differences in spontaneous tumor metastasis handling the animals for experimental procedures... 

Oh SJ, Ahn JY, Chung DH. Comparison of invariant NKT cells with conventional T cells by using gene set enrichment analysis (GSEA). Immune New. 2011 11 406-11. 

The best method to determine the success of antibody production to the minor components was made by two dimensional SDS-PAGE and immunoblotting (24). A comparison of the antisera (day 112 antisera) from the three groups demonstrated that the cascade immunization antisera detected a number of minor components (Figure 4C, arrows) which were not observed with the conventional or passive antisera (Figure 4B and D). It was clear from these results that the cascade antisera was far superior in its spectrum of antibody reactivity and, in fact, was comparable or superior in detection of ECPs to silver stain (Figure 4A). Although silver stain appeared to have an improved detection of certain low MW or basic proteins,... 

Immunoassays offer much potential for rapid screening and quantitative analysis of pesticides in food and environmental samples. However, despite this potential, the field is still dominated by conventional analytical approaches based upon chromatographic and spectrometric methods. We examine some technical barriers to more widespread adoption and utilization of immunoassays, including method development time, amount of information delivered and inexplicable sources of error. Examples are provided for paraquat in relation to exposure assessment in farmworkers and food residue analyses molinate in relation to low-level detection in surface waters and bentazon in relation to specificity and sensitivity requirements built in to the immunizing antigen. A comparison of enzyme-linked immunosorbent assay (ELISA) results with those obtained from conventional methods will illustrate technical implementation barriers and suggest ways to overcome them. 

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