Commercial assay kits optimization

Numerous biochemical tests are suitable as detection methods. The assay may be developed in-house. In this case, sufficient know-how shotdd be available to perform and optimize the respective tests. In addition, enzyme inhibition tests are well-suited for HPLC coupling. Even bioassays (e.g., toxicity tests) shotdd be suitable for this purpose. However, in this case, the toxicity of the solvent should be considered. Evaporation might be the best solution here as well. Users with less or even no experience of biochemical assays are not necessarily excluded from their application. Eor some analytical parameters, commercial test kits are available, which are not only useful for the analysis of the respective analytes in... 

The checkerboard approach is the common way a microtiter plate assay is developed and optimized. In terms of commercial kits, this approach could be used to change a kit component or, in the case of development kits (e.g., R + D systems Duo Sets ), to optimize the assay range for the specific requirements of the program. 

EnviroGard. The imaging ELISA values correlated well with the values obtained with the commercial kit The within-assay CV were between 6.4 and 11.4% while the day-to-day assay results were between 9,6 and 14.4%. A similar approach could be extended for simultaneous detection of multi-pesticide samples such as Atrazine, Simazine and 2,4-D employing the respective conjugate. The initial optimization of such assays are currently in progress (see Figure 4). 

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