Colloidal gold lectin labeling

Roth, J., and Binder, M. (1978) Colloidal gold, ferritin, and peroxidase as markers for electron microscopic double labeling lectin techniques./. Histochem. Cytochem. 26, 163. 

The up-to-date trends in using colloidal-gold markers for bioanalytical studies are as follows (i) to minimize the analyte substance amount (antigens, oligonucleotides, lectins, etc.) and (ii) to enhance the sensitivity of analysis. For instance, a sensitivity up to 60-70 ng L" has been reported [104] for an assay based on a simple replacing of the nitrocellulose membranes by microarrays combined with a photometrical reader. 11 should be emphasized that the microarray assay utilizes microliter volumes both for analyte probes and for labeled markers. Besides, the photometrical reader, together with the accessory equipment, allows for 384 probes to be analyzed within a short time period. 

Colloidal gold, ferritin, and peroxidase have been used as electron markers in the double labelling of a number of lectin-binding sites. ... 

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