Colloidal gold electron microscopy Transmission

Honisbenger, M., and Rosset, J. (1977) Colloidal gold, a useful marker for transmission and scanning electron microscopy./. Histochem. Cytochem. 25, 295. 

Horisberger, M. (1981) Colloidal gold a cytochemical marker for hght and fluorescent microscopy and for transmission and scanning electron microscopy, in Scanning Electron Microscopy II (Johari, O., ed.), SEM, Inc., AMF O Hare, Chicago, pp. 9-31. 

Fig. 6.1. HRTEM (High Resolution Transmission Electron Microscopy) of colloidal gold on carbon. Gold particles are viewed as dark points. Conditions [An] = 5 X 10 M, THPC/Au (mol moR ) = 2 (from [27])...

To give an idea of the monodispersity that can be achieved. Fig. 12.3 shows a transmission electron microscopy image of a colloidal gold sample prepared from... 

Key Words Transmission electron microscopy scanning electron microscopy Gram-positive Gram-negative immune labeling histochemistry colloidal gold quantum dots correlative microscopy fluorescence. 

Recent studies have shown that depletion-induced shape and size seleetion of colloidal particles has the potential to be a powerful enabling method to aehieve this in an effective way. For instance. Park et al. [280] reported the depletion-induced shape and size selection of gold rods and cubes. In Fig. 1.29 we show their transmission electron microscopy (TEM) images of gold rods (length L = ll nm, diameter D = 11 nm) and cubes (size is 20 nm). In (a) the synthesized mixture is shown, (b) depicts the sediment concentrated in golds rods and (c) is an image of the supernatant enriched in cubes. 

High resolution (transmission) electron microscopy (HREM) of the particles from this sol showed lattice detail ( 111 and 200 fringes) and particle structures (including, rather rarely, MTPs [9]) typical of colloidal gold microcrystallites. The UV-vis extinction spectrum of this boiled (coarsened) sol showed the expected strengthening of the plasmon feature due to the increase in particle size and concomitant decrease in finite-size effects [3] (see Fig. 2, curve B). 

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